Agar extraction and purification of R-phycoerythrin from Gracilaria tenuistipitata, and subsequent wastewater treatment by Ulva prolifera

doi:10.1016/j.algal.2020.101862

IOCAS-IR > 实验海洋生物学重点实验室

	Agar extraction and purification of R-phycoerythrin from Gracilaria tenuistipitata, and subsequent wastewater treatment by Ulva prolifera
	Zhao, Ping 1,2,3; Wang, Xulei1,2 ; Niu, Jianfeng 1,2; He, Linwen 1,2; Gu, Wenhui 1,2; Xie, Xiujun 1,2; Wu, Mingjiang 4; Wang, Guangce 1,2
	2020-05-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	47 页码:9
通讯作者	Wu, Mingjiang(wmj@wzu.edu.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
摘要	This study investigated the large-scale use of the red macroalga Gracilaria tenuistipitata to extract partially purified R-phycoerythrin in combination with the sequential extraction of agar. The main objective was to investigate the purification of R-PE, the extraction of agar and treatment of wastewater from the whole extraction process using the green macroalga Ulva prolifera. Results showed that the processing of 1 kg fresh biomass of G. tenuistipitata yielded a 30.34 mu g/g R-phycoerythrin (purification ratio > 4) with 13.83% extraction efficiency. Initial phycobiliprotein extraction had no effect on the agar yield. The physicochemical properties of the agar had industrial applications. This method of extraction reduced the water volume and alkali requirement compared with that from direct extraction from algae. In addition, the removal rates (mean +/- standard deviation) from wastewater of BOD, COD, total nitrogen, inorganic nitrogen, ammonia nitrogen, nitrate, nitrite, total phosphorus and inorganic phosphorus were 28.34 +/- 0.10%, 23.50 +/- 3.47%, 45.59 +/- 6.31%, 63.82 +/- 2.94%, 40.77 +/- 0.95%, 74.06 +/- 5.63%, 70.37 +/- 2.59%, 65.26 +/- 5.77%, and 72.40 +/- 5.43% after the treatment with 2 days respectively. This extraction and subsequently eco-friendly wastewater treatment method could have practical application in the agar industry.
关键词	Agar Gracilaria tenuistipitata R-phycoerythrin Ulva prolifera Wastewater treatment
DOI	10.1016/j.algal.2020.101862
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41706164] ; National Key the Natural Science Foundation of China[41876197]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000572122100002
出版者	ELSEVIER
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168896
专题	实验海洋生物学重点实验室
通讯作者	Wu, Mingjiang; Wang, Guangce
作者单位	1.Chinese Acad Sci, Key Lab Expt Marine Biol, Ctr Ocean Megasci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Wenzhou Univ, Coll Life & Environm Sci, Wenzhou, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhao, Ping,Wang, Xulei,Niu, Jianfeng,et al. Agar extraction and purification of R-phycoerythrin from Gracilaria tenuistipitata, and subsequent wastewater treatment by Ulva prolifera[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2020,47:9.
APA	Zhao, Ping.,Wang, Xulei.,Niu, Jianfeng.,He, Linwen.,Gu, Wenhui.,...&Wang, Guangce.(2020).Agar extraction and purification of R-phycoerythrin from Gracilaria tenuistipitata, and subsequent wastewater treatment by Ulva prolifera.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,47,9.
MLA	Zhao, Ping,et al."Agar extraction and purification of R-phycoerythrin from Gracilaria tenuistipitata, and subsequent wastewater treatment by Ulva prolifera".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 47(2020):9.

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