Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819)

doi:10.1111/j.1365-2109.2007.01683.x

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	Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819)
	Wang, Lingling ; Song, Linsheng ; Zhang, Huan ; Gao, Qiang ; Guo, Ximing ; Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
	2007-03-09
发表期刊	AQUACULTURE RESEARCH
ISSN	1355-557X
卷号	38 期号:4 页码:409-419
文章类型	Article
摘要	The bay scallop (Argopecten irradians irradians Lamarck 1819) has become one of the most important aquaculture species in China. Genetic improvement of cultured bay scallop can benefit greatly from a better understanding of its genome. In this study, we developed amplified fragment length polymorphisms (AFLPs) and simple sequence repeat markers from expressed sequence tags (EST-SSRs) for linkage analysis in bay scallop. Segregation of 390 AFLP and eight SSR markers was analysed in a mapping population of 97 progeny. Of the AFLP markers analysed, 326 segregated in the expected 1:1 Mendelian ratio, while the remaining 74 (or 19.0%) showed significant deviation, with 33 (44.6%) being deficient in heterozygotes (A/a). Among the eight polymorphic EST-SSR loci, one marker (12.5%) was found skewing from its expected Mendelian ratios. Eighteen per cent of the markers segregating from female parent were distorted compared with 21% of the markers segregating from male parent. The female map included 147 markers in 17 linkage groups (LGs) and covered 1892.4 cM of the genome. In the male map, totally 146 AFLP and SSR markers were grouped in 18 LGs spanning 1937.1 cM. The average inter-marker spacing in female and male map was 12.9 and 13.3 cM respectively. The AFLP and SSR markers were distributed evenly throughout the genome except for a few large gaps over 20 cM. Although preliminary, the genetic maps presented here provide a starting point for the mapping of the bay scallop genome.; The bay scallop (Argopecten irradians irradians Lamarck 1819) has become one of the most important aquaculture species in China. Genetic improvement of cultured bay scallop can benefit greatly from a better understanding of its genome. In this study, we developed amplified fragment length polymorphisms (AFLPs) and simple sequence repeat markers from expressed sequence tags (EST-SSRs) for linkage analysis in bay scallop. Segregation of 390 AFLP and eight SSR markers was analysed in a mapping population of 97 progeny. Of the AFLP markers analysed, 326 segregated in the expected 1:1 Mendelian ratio, while the remaining 74 (or 19.0%) showed significant deviation, with 33 (44.6%) being deficient in heterozygotes (A/a). Among the eight polymorphic EST-SSR loci, one marker (12.5%) was found skewing from its expected Mendelian ratios. Eighteen per cent of the markers segregating from female parent were distorted compared with 21% of the markers segregating from male parent. The female map included 147 markers in 17 linkage groups (LGs) and covered 1892.4 cM of the genome. In the male map, totally 146 AFLP and SSR markers were grouped in 18 LGs spanning 1937.1 cM. The average inter-marker spacing in female and male map was 12.9 and 13.3 cM respectively. The AFLP and SSR markers were distributed evenly throughout the genome except for a few large gaps over 20 cM. Although preliminary, the genetic maps presented here provide a starting point for the mapping of the bay scallop genome.
关键词	Linkage Map Aflp Ssr Scallop Argopecten Irradians Irradians
学科领域	Fisheries
DOI	10.1111/j.1365-2109.2007.01683.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000244716400009
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1687
专题	实验海洋生物学重点实验室
通讯作者	Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Qingdao 266071, Peoples R China 3.Rutgers State Univ, Inst Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ USA
推荐引用方式 GB/T 7714	Wang, Lingling,Song, Linsheng,Zhang, Huan,et al. Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819)[J]. AQUACULTURE RESEARCH,2007,38(4):409-419.
APA	Wang, Lingling,Song, Linsheng,Zhang, Huan,Gao, Qiang,Guo, Ximing,&Song, LS, Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2007).Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819).AQUACULTURE RESEARCH,38(4),409-419.
MLA	Wang, Lingling,et al."Genetic linkage map of bay scallop, Argopecten irradians irradians (Lamarck 1819)".AQUACULTURE RESEARCH 38.4(2007):409-419.

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