Variation of Amoebophrya community during bloom of Prorocentrum donghaiense Lu in coastal waters of the East China Sea

doi:10.1016/j.ecss.2020.106887

	Variation of Amoebophrya community during bloom of Prorocentrum donghaiense Lu in coastal waters of the East China Sea
	Chen, Tiantian1,2,3 ; Liu, Yun 1,2,3; Xu, Sha 1,2,4; Song, Shuqun 1,2,3; Li, Caiwen 1,2,3,4
	2020-09-30
发表期刊	ESTUARINE COASTAL AND SHELF SCIENCE
ISSN	0272-7714
卷号	243 页码:11
通讯作者	Li, Caiwen(cwli@qdio.ac.cn)
摘要	The parasitic dinoflagellates in the genus Amoebophrya infect and kill a number of free-living dinoflagellates. The parasitoid has the potential to regulate harmful dinoflagellate blooms due to its high offspring production rates and a certain degree of host specificity. The planktonic dinoflagellate Prorocentrum donghaiense Lu is a commonly observed harmful algal species in coastal waters of China, and limited studies have been conducted to explore the ecological roles of parasitism in association with bloom dynamics. Thus, the in situ occurrence, prevalence, and dynamics of Amoebophrya during the recurrent blooms of P. donghaiense were systemically studied via morphological observations and high-throughput sequencing. The parasitic infections were identified microscopically in six dinoflagellate taxa with prevalence up to 5.13%, while Amoebophrya infection in the bloomforming species P. donghaiense was rare (<0.01%), suggesting that P. donghaiense was free of the top-down control of Amoebophrya in the area. In addition, high-throughput sequencing of environmental DNA revealed that Amoebophrya co-dominated both the initiation and termination stages of the P. donghaiense bloom (up to 21.20% of OTU), but was less abundant during persistence of the bloom (3.70% of OTU). Further, the dynamics of Amoebophrya in the water column possessed similar patterns as revealed by qRT-PCR, in which high copy numbers of Amoebophrya (up to 3.60 x 10(9) copies L-1) were detected in the initiation and termination stages of the P. donghaiense bloom and low copy numbers (1.16 x 10(9) copies L-1) during persistence of the bloom. These findings manifested the paradoxical dynamics and complicated ecological strategies of Amoebophrya organisms during dinoflagellate blooms that need further study.
关键词	Harmful algal blooms Dinoflagellate Parasitism High-throughput sequencing qRT-PCR
DOI	10.1016/j.ecss.2020.106887
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China (NSFC)[41906122] ; National Natural Science Foundation of China (NSFC)[48176120] ; National Natural Science Foundation of China (NSFC)[41606128] ; China Postdoctoral Science Foundation[2018M642709] ; National Key R&D Program of China[2017YFC1404300] ; Marine S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0504-2]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Marine & Freshwater Biology ; Oceanography
WOS记录号	WOS:000570081600003
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168787
专题	海洋生态与环境科学重点实验室
通讯作者	Li, Caiwen
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingda 266200, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
通讯作者单位	海洋生态与环境科学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Chen, Tiantian,Liu, Yun,Xu, Sha,et al. Variation of Amoebophrya community during bloom of Prorocentrum donghaiense Lu in coastal waters of the East China Sea[J]. ESTUARINE COASTAL AND SHELF SCIENCE,2020,243:11.
APA	Chen, Tiantian,Liu, Yun,Xu, Sha,Song, Shuqun,&Li, Caiwen.(2020).Variation of Amoebophrya community during bloom of Prorocentrum donghaiense Lu in coastal waters of the East China Sea.ESTUARINE COASTAL AND SHELF SCIENCE,243,11.
MLA	Chen, Tiantian,et al."Variation of Amoebophrya community during bloom of Prorocentrum donghaiense Lu in coastal waters of the East China Sea".ESTUARINE COASTAL AND SHELF SCIENCE 243(2020):11.

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