Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| IL-34 regulates the inflammatory response and anti-bacterial immune defense of Japanese flounder Paralichthys olivaceus | |
Yu, Chao1,2,3; Zhang, Peng1,2,3 ; Zhang, Teng-fei1,2,3; Sun, Li1,2
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| 2020-09-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| Volume | 104Pages:228-236 |
| Corresponding Author | Sun, Li(lsun@qdio.ac.cn) |
| Abstract | Interleukin (IL)-34 is a relatively recently discovered cytokine with pleiotropic effects on various cellular activities, including immune response. In fish, the knowledge on the function of IL-34 is limited. In the present work, we investigated the function of Japanese flounder Paralichthys olivaceus IL-34 (PoIL-34) in association with inflammation and immune defense. PoIL-34 possesses the conserved structure of IL-34 superfamily and shares 21.52% sequence identity with murine IL-34. PoIL-34 expression was detected in a wide range of tissues of flounder, in particular intestine, and was regulated to a significant extent by bacterial infection in a time-dependent fashion. In vitro studies showed that recombinant PoIL-34 (rPoIL-34) bound peripheral blood leukocytes (PBLs) and promoted ROS production, acid phosphatase activity, and cellular resistance against bacterial infection. At the molecular level, rPoIL-34 enhanced the expressions of inflammatory cytokines and specific JAK and STAT genes. Similar stimulatory effects of rPoIL-34 were observed in vivo. When PoIL-34 was overexpressed in flounder, the expressions of pro- and anti-inflammatory mediators were significantly affected in a tissue-dependent manner, which correlated with an augmented ability of the fish to eliminate invading pathogens from tissues. Together, these results indicated that PoIL-34 regulated inflammatory response probably via specific JAK/STAT pathways and had a significant influence on the immune defense of flounder against bacterial infection. |
| Keyword | Paralichthys olivaceus Interleukin 34 Inflammatory response Peripheral blood leukocytes Immune defense |
| DOI | 10.1016/j.fsi.2020.05.073 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Key Research & Development Project of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000564750000013 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/168538 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Sun, Li |
| Affiliation | 1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| First Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Yu, Chao,Zhang, Peng,Zhang, Teng-fei,et al. IL-34 regulates the inflammatory response and anti-bacterial immune defense of Japanese flounder Paralichthys olivaceus[J]. FISH & SHELLFISH IMMUNOLOGY,2020,104:228-236. |
| APA | Yu, Chao,Zhang, Peng,Zhang, Teng-fei,&Sun, Li.(2020).IL-34 regulates the inflammatory response and anti-bacterial immune defense of Japanese flounder Paralichthys olivaceus.FISH & SHELLFISH IMMUNOLOGY,104,228-236. |
| MLA | Yu, Chao,et al."IL-34 regulates the inflammatory response and anti-bacterial immune defense of Japanese flounder Paralichthys olivaceus".FISH & SHELLFISH IMMUNOLOGY 104(2020):228-236. |
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| IL-34 regulates the （2759KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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