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Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics
Yang, Weihong1,2,3; Huan, Pin1,2,3; Liu, Baozhong1,2,3
2020-08-19
Source PublicationBMC DEVELOPMENTAL BIOLOGY
ISSN1471-213X
Volume20Issue:1Pages:10
Corresponding AuthorHuan, Pin(huanpin@qdio.ac.cn)
AbstractBackground The morphogenesis of the shell field is an essential step of molluscan shell formation, which exhibits both conserved features and interlineage variations. As one major gastropod lineage, the patellogastropods show different characters in its shell field morphogenesis compared to other gastropods (e.g., the pulmonate gastropodLymnaea stagnalis), likely related to its epibolic gastrulation. The investigation on the shell field morphogenesis of patellogastropods would be useful to reveal the lineage-specific characters in the process and explore the deep conservation among different molluscan lineages. Results We investigated the early shell field morphogenesis in the patellogastropodLottia goshimaiusing multiple techniques. Electron microscopy revealed distinct morphological characters for the central and peripheral cells of the characteristic rosette-like shell field. Gene expression analysis and F-actin staining suggested that the shell field morphogenesis in this species predominantly relied on cell movement and F-actin dynamics, while BrdU assay revealed that cell proliferation contributed little to the process. We found constant contacts between ectodermal and meso/endodermal tissues during the early stages of shell field morphogenesis, which did not support the induction of shell field by endodermal tissues in general, but a potential stage-specific induction was indicated. Conclusions Our results emphasize the roles of cell movement and F-actin dynamics during the morphogenesis of the shell field inLo. goshimai, and suggest potential regulators such as diffusible factors and F-actin modulators. These findings reflect the differences in shell field morphogenesis of different gastropods, and add to the knowledge of molluscan larval shell formation.
KeywordCell movement F-actin dynamics Mollusk Morphogenesis Shell field Shell-formation gene
DOI10.1186/s12861-020-00223-3
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41776157] ; National Key R&D Program of China[2018YFD0900104] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-1]
WOS Research AreaDevelopmental Biology
WOS SubjectDevelopmental Biology
WOS IDWOS:000565127900001
PublisherBMC
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168448
Collection实验海洋生物学重点实验室
Corresponding AuthorHuan, Pin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Weihong,Huan, Pin,Liu, Baozhong. Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics[J]. BMC DEVELOPMENTAL BIOLOGY,2020,20(1):10.
APA Yang, Weihong,Huan, Pin,&Liu, Baozhong.(2020).Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics.BMC DEVELOPMENTAL BIOLOGY,20(1),10.
MLA Yang, Weihong,et al."Early shell field morphogenesis of a patellogastropod mollusk predominantly relies on cell movement and F-actin dynamics".BMC DEVELOPMENTAL BIOLOGY 20.1(2020):10.
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