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Sex-Dependent RNA Editing andN6-adenosine RNA Methylation Profiling in the Gonads of a Fish, the Olive Flounder (Paralichthys olivaceus)
Wang, Lijuan1,2; Wu, Zhihao1,2; Zou, Congcong1,2,3; Liang, Shaoshuai1,2; Zou, Yuxia1,2; Liu, Yan1,2; You, Feng1,2
2020-08-05
Source PublicationFRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
ISSN2296-634X
Volume8Pages:21
AbstractAdenosine-to-inosine (A-to-I) editing andN6-methyladenosine (m6A) are two of the most abundant RNA modifications. Here, we examined the characteristics of the RNA editing and transcriptome-wide m6A modification profile in the gonads of the olive flounder,Paralichthys olivaceus, an important maricultured fish in Asia. The gonadal differentiation and development of the flounder are controlled by genetic as well as environmental factors, and the epigenetic mechanism may play an important role. In total, 742 RNA editing events were identified, 459 of which caused A to I conversion. Most A-to-I sites were located in 3 ' UTRs, while 61 were detected in coding regions (CDs). The number of editing sites in the testis was higher than that in the ovary. Transcriptome-wide analyses showed that more than one-half of the transcribed genes presented an m6A modification in the flounder gonads, and approximately 60% of the differentially expressed genes (DEGs) between the testis and ovary appeared to be negatively correlated with m6A methylation enrichment. Further analyses revealed that the mRNA expression of some sex-related genes (e.g.,dmrt1andamh) in the gonads may be regulated by changes in mRNA m6A enrichment. Functional enrichment analysis indicated that the RNA editing and m6A modifications were enriched in several canonical pathways (e.g., Wnt and MAPK signaling pathways) in fish gonads and in some pathways whose roles have not been investigated in relation to fish sex differentiation and gonadal development (e.g., PPAR and RNA degradation pathways). There were 125 genes that were modified by both A-to-I editing and m6A, but the two types of modifications mostly occurred at different sites. Our results suggested that the presence of sex-specific RNA modifications may be involved in the regulation of gonadal development and gametogenesis.
KeywordRNA editing N6-methyladenosine ovary testis Paralichthys olivaceus
DOI10.3389/fcell.2020.00751
Indexed BySCI
Language英语
Funding ProjectNational Infrastructure of Fishery Germplasm Resources[2017DKA30470] ; National Natural Science Foundation of China[31772834] ; National Key R&D Program of China[2018YFD0900202] ; National Natural Science Foundation of China[31702337] ; Youth Research Fund of Marine Biology and Biotechnology Laboratory, Pilot National Laboratory for Marine Science and Technology (Qingdao)[YQ2018NO01] ; Key R&D (public welfare) program of Shandong Province[2019GHY112005]
WOS Research AreaCell Biology ; Developmental Biology
WOS SubjectCell Biology ; Developmental Biology
WOS IDWOS:000563260500001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/168343
Collection实验海洋生物学重点实验室
Corresponding AuthorYou, Feng
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Lijuan,Wu, Zhihao,Zou, Congcong,et al. Sex-Dependent RNA Editing andN6-adenosine RNA Methylation Profiling in the Gonads of a Fish, the Olive Flounder (Paralichthys olivaceus)[J]. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,2020,8:21.
APA Wang, Lijuan.,Wu, Zhihao.,Zou, Congcong.,Liang, Shaoshuai.,Zou, Yuxia.,...&You, Feng.(2020).Sex-Dependent RNA Editing andN6-adenosine RNA Methylation Profiling in the Gonads of a Fish, the Olive Flounder (Paralichthys olivaceus).FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY,8,21.
MLA Wang, Lijuan,et al."Sex-Dependent RNA Editing andN6-adenosine RNA Methylation Profiling in the Gonads of a Fish, the Olive Flounder (Paralichthys olivaceus)".FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 8(2020):21.
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