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Novel insights into type 2 diacylglycerol acyltransferases in microalgaMyrmecia incisa
Liu, Wei1,2,3; Sun, Zheng1,2,3; Chen, Chun-Xiu1,2,3; Liu, Jian-Guo4; Zhou, Zhi-Gang1,2,3
2020-03-12
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Pages11
AbstractThe green algaMyrmecia incisais rich in arachidonic acid (ArA), which mainly stores in lipid droplets in the form of triacylglycerol (TAG). In the de novo biosynthesis of TAG, diacylglycerol acyltransferase (DGAT) catalyzes the final and committed step as a rate-limiting enzyme. Previously, our lab has reported the first DGAT inM. incisa, and in this study, a new DGAT was further identified. Through homology search against our transcriptome database and annotation, a new contig corresponding to the putative DGAT2 was targeted, whose full-length cDNA was cloned by RACE and designated asMiDGAT2C. For this new MiDGAT2C, bioinformatics analyses revealed the presence of multiple conserved amino acid residues and sequences. Its function to synthesize TAG was determined by complementation assay using the TAG-deficient yeast system. Besides, MiDGAT2C was compared with other MiDGATs with respect to their substrate preference. Results of fatty acid feeding and in vitro enzymatic assays indicated diverse substrate specificities among MiDGAT members. While MiDGAT2C showed no activity on ArA, MiDGAT2A made the most contribution to incorporate ArA into TAG. Findings of this study expand our knowledge on the molecular basis of DGAT, shedding more light onM. incisaas a promising feedstock for ArA-rich TAG.
KeywordTriacylglycerol Myrmecia incisa Chlorophyta Cloning Functional complementation Substrate preference
DOI10.1007/s10811-020-02071-x
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China (NSFC)[31772821] ; NSFC-Shandong Joint Fund[U1706209] ; State Double FirstClass Discipline Project of Aquaculture
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000562925900001
PublisherSPRINGER
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/168307
Collection中国科学院海洋研究所
Corresponding AuthorZhou, Zhi-Gang
Affiliation1.Shanghai Ocean Univ, Minist Educ, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai 201306, Peoples R China
2.Shanghai Ocean Univ, Minist Sci & Technol, Int Res Ctr Marine Biosci, Shanghai 201306, Peoples R China
3.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Liu, Wei,Sun, Zheng,Chen, Chun-Xiu,et al. Novel insights into type 2 diacylglycerol acyltransferases in microalgaMyrmecia incisa[J]. JOURNAL OF APPLIED PHYCOLOGY,2020:11.
APA Liu, Wei,Sun, Zheng,Chen, Chun-Xiu,Liu, Jian-Guo,&Zhou, Zhi-Gang.(2020).Novel insights into type 2 diacylglycerol acyltransferases in microalgaMyrmecia incisa.JOURNAL OF APPLIED PHYCOLOGY,11.
MLA Liu, Wei,et al."Novel insights into type 2 diacylglycerol acyltransferases in microalgaMyrmecia incisa".JOURNAL OF APPLIED PHYCOLOGY (2020):11.
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