IOCAS-IR
Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives
Sun, Shenmei1,2; Ding, Yamei3; Liu, Min1; Xian, Mo1; Zhao, Guang1,4
2020-07-23
Source PublicationFRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
ISSN2296-4185
Volume8Pages:13
Corresponding AuthorZhao, Guang(zhaoguang@sdu.edu.cn)
AbstractPoly(3-hydroxybutyrate) (PHB) is a biodegradable and biocompatible thermoplastic, and synthesized from the central metabolite acetyl-CoA. The acetyl-CoA synthesis from glucose presents low atomic economy due to the release of CO(2)in pyruvate decarboxylation. As ethanol and acetate can be converted into acetyl-CoA directly, they were used as carbon source for PHB production in this study. The reductase mutant AdhE A267T/E568K was introduced intoEscherichia colito enable growth on ethanol, and acetate utilization was improved by overexpression of acetyl-CoA synthetase ACS. Comparison of the PHB production using glucose, ethanol or acetate as sole carbon source showed that the production and yield from ethanol was much higher than those from glucose and acetate, and metabolome analysis revealed the differences in metabolism of glucose, ethanol and acetate. Furthermore, other acetyl-CoA derived chemicals including 3-hydroxypropionate and phloroglucinol were produced from those three feedstocks, and similar results were achieved, suggesting that ethanol could be a suitable carbon source for the production of acetyl-CoA derivatives.
Keywordcarbon source glucose ethanol acetate acetyl-CoA bioproduction
DOI10.3389/fbioe.2020.00833
Indexed BySCI
Language英语
Funding ProjectNSFC[31722001] ; NSFC[31670089] ; Natural Science Foundation of Shandong Province[JQ201707] ; National Defense Science and Technology Innovation Zone Foundation of China ; Defense Industrial Technology Development Program[JCKY2018130B005] ; Shandong University
WOS Research AreaBiotechnology & Applied Microbiology ; Science & Technology - Other Topics
WOS SubjectBiotechnology & Applied Microbiology ; Multidisciplinary Sciences
WOS IDWOS:000560293700001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168187
Collection中国科学院海洋研究所
Corresponding AuthorZhao, Guang
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
4.Shandong Univ, State Key Lab Microbial Technol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Sun, Shenmei,Ding, Yamei,Liu, Min,et al. Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives[J]. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,2020,8:13.
APA Sun, Shenmei,Ding, Yamei,Liu, Min,Xian, Mo,&Zhao, Guang.(2020).Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives.FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY,8,13.
MLA Sun, Shenmei,et al."Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives".FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 8(2020):13.
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