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Antioxidant enzymes and the mitochondrial alternative oxidase pathway play important roles in chilling tolerance of Haematococcus pluvialis at the green motile stage
Zhang, Chunhui1; Li, Runzhi1; Zhu, Qin1; Hang, Wei1; Zhang, Hongjiang1; Cui, Hongli1; Ji, Chunli1; Zhang, Litao2; Chen, Fangjian3
2020-09-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume50Pages:8
AbstractDuring outdoor large-scale cultivation, Haematococcus pluvialis cells, especially at the green motile stage, are frequently exposed to chilling stress in winter. However, the physiological responses and adaptation mechanisms to chilling in H. pluvialis have not been characterized. In order to better understand the chilling tolerance mechanisms of H. pluvialis in the green motile stage, the responses of photosynthetic characteristics and photo-protective mechanisms to chilling were investigated. Chilling stress significantly decreased the activities of key enzymes (ribulose-1,5-bisphosphate carboxylase/oxygenase and NADP glyceraldehyde-3-phosphate dehydrogenase) in photosynthetic carbon assimilation, which would cause the accumulation of excess reducing equivalents and an imbalance of light absorption and energy utilization, leading to more severe photoinhibition. After chilling treatment, there was no induction of cyclic electron flow, non-photochemical quenching, fluorescence emission, or catalase, indicating these factors do not protect H. pluvialis from photoinhibition. However, chilling significantly enhanced the activities of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, dehydroascorbate reductase, and glutathione reductase), which efficiently dissipated excess electrons generated by photosynthetic linear electron flow (LEF). The malate-oxaloacetate shuttle was activated and the mitochondrial alternative oxidase (AOX) pathway was significantly up-regulated after chilling, indicating the transport of excess reducing equivalents generated by photosynthetic LEF to cytosol and mitochondria and oxidization by the AOX pathway, the mitochondrial non-phosphorylating pathway. As a result, the LEF was not inhibited by negative feedback and the H. pluvialis cells were well protected under chilling stress in the light, with no change in the effective quantum yield of PSII (FPSII) and the yield of non-regulated energy dissipation of PSII (FNO). The results indicate that antioxidant enzymes and the mitochondrial AOX pathway play important roles in protecting H. pluvialis at the green motile stage against chilling stress.
KeywordChilling tolerance Microalgae Photoinhibition Photoprotection Reducing equivalents
DOI10.1016/j.algal.2020.102003
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFE0107200] ; National Natural Science Foundation of China[31872588] ; Shandong Provincial Natural Science Foundation, China[ZR2019BC057] ; Doctoral Incentive Funding Research Project of Shanxi Province[SXYBKY2019037]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000558684000002
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/168184
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Runzhi; Zhang, Litao
Affiliation1.Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Taigu 030801, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Chunhui,Li, Runzhi,Zhu, Qin,et al. Antioxidant enzymes and the mitochondrial alternative oxidase pathway play important roles in chilling tolerance of Haematococcus pluvialis at the green motile stage[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2020,50:8.
APA Zhang, Chunhui.,Li, Runzhi.,Zhu, Qin.,Hang, Wei.,Zhang, Hongjiang.,...&Chen, Fangjian.(2020).Antioxidant enzymes and the mitochondrial alternative oxidase pathway play important roles in chilling tolerance of Haematococcus pluvialis at the green motile stage.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,50,8.
MLA Zhang, Chunhui,et al."Antioxidant enzymes and the mitochondrial alternative oxidase pathway play important roles in chilling tolerance of Haematococcus pluvialis at the green motile stage".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 50(2020):8.
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