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A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas
Liu, Conghui1,2,3; Yang, Chuanyan1,3,4; Wang, Mengqiang2; Jiang, Shuai2; Yi, Qilin1,3,4; Wang, Weilin2,3,4; Wang, Lingling1,3,4; Song, Linsheng1,3
2020-07-22
Source PublicationFRONTIERS IN IMMUNOLOGY
ISSN1664-3224
Volume11Pages:12
Corresponding AuthorWang, Lingling(wanglingling@dlou.edu.cn) ; Song, Linsheng(lshsong@dlou.edu.cn)
AbstractCluster of differentiation 63 (CD63), a four-transmembrane glycoprotein in the subfamily of tetraspanin, has been widely recognized as a gateway from the infection of foreign invaders to the immune defense of hosts. Its role in Pacific oysterCrassostrea gigasis, however, yet to be discovered. This work makes contributions by identifyingCgCD63H, a CD63 homolog with four transmembrane domains and one conservative CCG motif, and establishing its role as a receptor that participates in immune recognition and hemocyte phagocytosis. The presence ofCgCD63H messenger RNA (mRNA) in hepatopancreas, labial palps, gill, and hemocytes is confirmed. The expression level of mRNA in hemocytes is found significantly (p< 0.01) upregulated after the injection ofVibrio splendidus.CgCD63H protein, typically distributed over the plasma membrane of oyster hemocytes, is recruited to theYarrowia lipolytica-containing phagosomes after the stimulation ofY. lipolytica. The recombinantCgCD63H protein expresses binding capacity to glucan (GLU), peptidoglycan (PGN), and lipopolysaccharide (LPS) in the presence of lyophilized hemolymph. The phagocytic rate of hemocytes towardV. splendidusandY. lipolyticais significantly inhibited (p< 0.01) after incubation with anti-CgCD63H antibody. Our work further suggests thatCgCD63H functions as a receptor involved in the immune recognition and hemocyte phagocytosis against invading pathogen, which can be a marker candidate for the hemocyte typing inC. gigas.
Keywordtetraspanin phagosomes recruitment receptor innate immune response Crassostrea gigas
DOI10.3389/fimmu.2020.01379
Indexed BySCI
Language英语
Funding ProjectNational Key RD Program[2018YFD0900606] ; National Science Foundation of China[U1706204] ; National Science Foundation of China[41961124009] ; Modern Agro-Industry Technology Research System[CARS-49] ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research from the Ministry of Agriculture and Rural Affairs in China ; AoShan Talents Cultivation Program - Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Liaoning Climbing Scholar ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University ; [XLYC1902012]
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000559341100001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168181
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Lingling; Song, Linsheng
Affiliation1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian, Peoples R China
4.Dalian Ocean Univ, Dalian Key Lab Aquat Anim Dis Prevent & Control, Dalian, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Conghui,Yang, Chuanyan,Wang, Mengqiang,et al. A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas[J]. FRONTIERS IN IMMUNOLOGY,2020,11:12.
APA Liu, Conghui.,Yang, Chuanyan.,Wang, Mengqiang.,Jiang, Shuai.,Yi, Qilin.,...&Song, Linsheng.(2020).A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas.FRONTIERS IN IMMUNOLOGY,11,12.
MLA Liu, Conghui,et al."A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of OysterCrassostrea gigas".FRONTIERS IN IMMUNOLOGY 11(2020):12.
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