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CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas)
Tang, Xueying1,2; Huang, Baoyu1,3,4; Lin, Siheng1,3; Wang, Wei1,3,4,5; Zhang, Guofan1,3,4,5; Li, Li1,4,5,6
2020-07-14
Source PublicationFRONTIERS IN IMMUNOLOGY
ISSN1664-3224
Volume11Pages:12
Corresponding AuthorLi, Li(lili@qdio.ac.cn)
AbstractOstreid herpesvirus-1 microvariant (OsHV-1 mu Var) is considered a major infectious microbe that can reduce the survival of natural or cultured oysters in summer. Because they lack an adaptive immune system, oysters are dependent on their innate immune systems to fight pathogens. The duplication and functional divergence of innate immune genes in the oyster have been studied, but the contribution of molecular mechanisms underlying innate immunity remains to be defined. Here, we identified the interacting proteins associated withCrassostrea gigasToll-like receptors (CgTLR) using a yeast two-hybrid (Y2H) screening system. A total of eight proteins were identified that could interact with CgTLR. Three of these appeared at least four times in the screening and were related to MyD88. Two genes encoding these MyD88-like proteins, CgMyD88-1 and CgMyD88-2, possessed typical death and TIR domains. The third gene encoding an MyD88-like protein possessed only a TIR domain, and we named it CgMyD88s. CgMyD88s interacted only with CgTLR, but not CgMyD88-1 or CgMyD88-2. Both CgMyD88-1 and CgMyD88-2 mRNAs were upregulated after OsHV-1 mu Var infection, whereas the expression of CgMyD88s decreased. When overexpressed in HEK293T cells, CgMyD88-1 and CgMyD88-2 activated an NF-kappa B reporter, whereas CgMyD88s impaired activation induced by CgMyD88-1 or CgMyD88-2. Intriguingly, the silencing of CgMyD88s using double-stranded RNA (dsRNA)-mediated RNA interference increased the expression of CgMyD88-1 and CgMyD88-2. Taken together, our results revealed that CgMyD88-1, CgMyD88-2, and CgMyD88s may all participate in the TLR-mediated innate immune pathway and that CgMyD88s served as a plug to avoid oysters from excessive inflammatory response during OsHV-1 mu Var infections.
Keywordinnate immunity OsHV-1 mu Var Crassostrea gigas TLR MyD88
DOI10.3389/fimmu.2020.01247
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0900304] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; Key Deployment Project of Center for Ocean Mega-Research of Sciences[COMS2019Q06] ; Earmarked Fund for China Agriculture Research System[CARS-49] ; Scientific Research Staring Foundation of Minnan Normal University[4206-L21829]
WOS Research AreaImmunology
WOS SubjectImmunology
WOS IDWOS:000556583000001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168130
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Li
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Minnan Normal Univ, Dept Biol Sci & Biotechnol, Zhangzhou, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
5.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China
6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Tang, Xueying,Huang, Baoyu,Lin, Siheng,et al. CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas)[J]. FRONTIERS IN IMMUNOLOGY,2020,11:12.
APA Tang, Xueying,Huang, Baoyu,Lin, Siheng,Wang, Wei,Zhang, Guofan,&Li, Li.(2020).CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas).FRONTIERS IN IMMUNOLOGY,11,12.
MLA Tang, Xueying,et al."CgMyD88s Serves as an Innate Immune System Plug During Ostreid Herpesvirus 1 Infection in the Pacific Oyster (Crassostrea gigas)".FRONTIERS IN IMMUNOLOGY 11(2020):12.
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