Molecular and Functional Diversity of Crustin-Like Genes in the ShrimpLitopenaeus vannamei

doi:10.3390/md18070361

IOCAS-IR > 实验海洋生物学重点实验室

	Molecular and Functional Diversity of Crustin-Like Genes in the ShrimpLitopenaeus vannamei
	Li, Shihao 1,2,3; Lv, Xinjia1,4 ; Yu, Yang1,2,3 ; Zhang, Xiaojun 1,2,3; Li, Fuhua 1,2,3,5
	2020-07-01
发表期刊	MARINE DRUGS
卷号	18 期号:7 页码:17
通讯作者	Li, Fuhua(fhli@qdio.ac.cn)
摘要	Crustins are crustacean cationic cysteine-rich antimicrobial peptides that contain one or two whey acidic protein (WAP) domain(s) at the carboxyl terminus and mainly show antimicrobial and/or proteinase inhibitory activities. Here, we performed genome and transcriptome screening and identified 34 full-length crustin-like encoding genes inLitopenaeus vannamei. Multiple sequence analysis of the deduced mature peptides revealed that these putative crustins included 10 type Ia, two type Ib, one type Ic, 11 type IIa, three type IIb, four type III, one type IV, one type VI, and one type VII. These putative crustins were clustered into different groups. Phylogenetic analysis, considering their domain composition, showed that different types of crustin-like genes in crustaceans might be originated from the WAP core region, along with sequence insertion, duplication, deletion, and amino acid substitution. Tissue distribution analysis suggested that most crustin-like genes were mainly detected in immune-related tissues while several crustin-like genes exhibited tissue-specific expression patterns. Quantitative PCR analysis on 15 selected crustin-like genes showed that most of them were apparently upregulated afterVibrio parahaemolyticusor white spot syndrome virus (WSSV) infection. One type Ib crustin-like gene, mainly expressed in the ovary, showed the highest expression levels before the gastrula stage and was hardly detected after the limb bud stage, suggesting that it was a maternal immune effector. Collectively, the present data revealed the molecular and functional diversity of crustins and their potential evolutionary routes in crustaceans.
关键词	crustin sequence diversity immunological function Litopenaeus vannamei
DOI	10.3390/md18070361
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[41776158] ; China Agriculture Research system-48 (CARS-48)
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal
WOS记录号	WOS:000557297400001
出版者	MDPI
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/168118
专题	实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
第一作者单位	实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心
通讯作者单位	实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Li, Shihao,Lv, Xinjia,Yu, Yang,et al. Molecular and Functional Diversity of Crustin-Like Genes in the ShrimpLitopenaeus vannamei[J]. MARINE DRUGS,2020,18(7):17.
APA	Li, Shihao,Lv, Xinjia,Yu, Yang,Zhang, Xiaojun,&Li, Fuhua.(2020).Molecular and Functional Diversity of Crustin-Like Genes in the ShrimpLitopenaeus vannamei.MARINE DRUGS,18(7),17.
MLA	Li, Shihao,et al."Molecular and Functional Diversity of Crustin-Like Genes in the ShrimpLitopenaeus vannamei".MARINE DRUGS 18.7(2020):17.

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Molecular and Functi（4033KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览