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Molecular and Functional Diversity of Crustin-Like Genes in the ShrimpLitopenaeus vannamei
Li, Shihao1,2,3; Lv, Xinjia1,4; Yu, Yang1,2,3; Zhang, Xiaojun1,2,3; Li, Fuhua1,2,3,5
2020-07-01
Source PublicationMARINE DRUGS
Volume18Issue:7Pages:17
Corresponding AuthorLi, Fuhua(fhli@qdio.ac.cn)
AbstractCrustins are crustacean cationic cysteine-rich antimicrobial peptides that contain one or two whey acidic protein (WAP) domain(s) at the carboxyl terminus and mainly show antimicrobial and/or proteinase inhibitory activities. Here, we performed genome and transcriptome screening and identified 34 full-length crustin-like encoding genes inLitopenaeus vannamei. Multiple sequence analysis of the deduced mature peptides revealed that these putative crustins included 10 type Ia, two type Ib, one type Ic, 11 type IIa, three type IIb, four type III, one type IV, one type VI, and one type VII. These putative crustins were clustered into different groups. Phylogenetic analysis, considering their domain composition, showed that different types of crustin-like genes in crustaceans might be originated from the WAP core region, along with sequence insertion, duplication, deletion, and amino acid substitution. Tissue distribution analysis suggested that most crustin-like genes were mainly detected in immune-related tissues while several crustin-like genes exhibited tissue-specific expression patterns. Quantitative PCR analysis on 15 selected crustin-like genes showed that most of them were apparently upregulated afterVibrio parahaemolyticusor white spot syndrome virus (WSSV) infection. One type Ib crustin-like gene, mainly expressed in the ovary, showed the highest expression levels before the gastrula stage and was hardly detected after the limb bud stage, suggesting that it was a maternal immune effector. Collectively, the present data revealed the molecular and functional diversity of crustins and their potential evolutionary routes in crustaceans.
Keywordcrustin sequence diversity immunological function Litopenaeus vannamei
DOI10.3390/md18070361
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[41776158] ; China Agriculture Research system-48 (CARS-48)
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000557297400001
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168118
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Fuhua
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Chinese Acad Sci, Innovat Seed Design, Wuhan 430072, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Shihao,Lv, Xinjia,Yu, Yang,et al. Molecular and Functional Diversity of Crustin-Like Genes in the ShrimpLitopenaeus vannamei[J]. MARINE DRUGS,2020,18(7):17.
APA Li, Shihao,Lv, Xinjia,Yu, Yang,Zhang, Xiaojun,&Li, Fuhua.(2020).Molecular and Functional Diversity of Crustin-Like Genes in the ShrimpLitopenaeus vannamei.MARINE DRUGS,18(7),17.
MLA Li, Shihao,et al."Molecular and Functional Diversity of Crustin-Like Genes in the ShrimpLitopenaeus vannamei".MARINE DRUGS 18.7(2020):17.
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