IOCAS-IR  > 实验海洋生物学重点实验室
Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus
Wang, Wenxiang1,2,3; Liang, Shaoshuai1,2; Zou, Yuxia1,2; Wu, Zhihao1,2; Wang, Lijuan1,2; Liu, Yan1,2; You, Feng1,2
2020-09-10
Source PublicationGENE
ISSN0378-1119
Volume755Pages:9
AbstractThe olive flounder Paralichthys olivaceus, an important marine fish, shows gender differences in growth. The mechanism on its gonadal differentiation direction affected with exogenous factors still needs to be clarified. The anti-Mullerian hormone (amh) gene is involved in fish testicular differentiation and maintenance. The aim of this study was to investigate the expression of the flounder amh in tissues and the gonads. The quantitative expression analysis results showed that it was highly expressed in the testis, especially in the testis at stages I - IV (P < 0.05). Also, amh was detected in Sertoli cells surrounding spermatogonia and peripheral seminiferous lobule of the testis with in situ hybridization (ISH) and immunohistochemistry (IHC). During the differentiation period, the amh expression in the testis of the tamoxifen treatment group (100 ppm) was higher than that in the ovary of the 17 beta-estradiol (E2, 5 ppm) group, and the expression levels of amh during process of the male differentiation in the tamoxifen group were higher than those in the 17 alpha-methyltestosterone (MT, 5 ppm) group (P < 0.05). ISH results also exhibited that amh was expressed in the somatic cells that surrounded the germ cells of juvenile flounder similar to adult ones. Furthermore, the flounder gonads in the tamoxifen group maintained more germ cells and somatic cells than those in the MT group from 20 to 80 mm total length (TL). Especially, at 60 and 80 mm TL, the numbers of germ and somatic cells in the tamoxifen group were significantly higher than those in the MT group (P < 0.05). In summary, amh might initiate the process of testicular differentiation, and is involved in the early development and maintenance of testis.
KeywordOlive flounder Paralichthys olivaceus Amh Expression Testicular differentiation and development Somatic cell
DOI10.1016/j.gene.2020.144906
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31872558] ; National Natural Science Foundation of China[31702337] ; National Key R&D Program of China[2018YFD0900202] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000555786400011
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/168109
Collection实验海洋生物学重点实验室
Corresponding AuthorYou, Feng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Wenxiang,Liang, Shaoshuai,Zou, Yuxia,et al. Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus[J]. GENE,2020,755:9.
APA Wang, Wenxiang.,Liang, Shaoshuai.,Zou, Yuxia.,Wu, Zhihao.,Wang, Lijuan.,...&You, Feng.(2020).Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus.GENE,755,9.
MLA Wang, Wenxiang,et al."Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus".GENE 755(2020):9.
Files in This Item:
File Name/Size DocType Version Access License
Amh dominant express(2594KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Wenxiang]'s Articles
[Liang, Shaoshuai]'s Articles
[Zou, Yuxia]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Wenxiang]'s Articles
[Liang, Shaoshuai]'s Articles
[Zou, Yuxia]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Wenxiang]'s Articles
[Liang, Shaoshuai]'s Articles
[Zou, Yuxia]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Amh dominant expression in Sertoli cells during the testicular differentiation and development stages in the olive flounder Paralichthys olivaceus.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.