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Effects of Sudden Drop in Salinity on Osmotic Pressure Regulation and Antioxidant Defense Mechanism ofScapharca subcrenata
Zhang Mo1,2; Li Li3; Liu Ying4; Gao Xiaolong1
2020-07-17
Source PublicationFRONTIERS IN PHYSIOLOGY
ISSN1664-042X
Volume11Pages:14
Corresponding AuthorGao Xiaolong(xlgao@xmu.edu.cn)
AbstractSalinity is an important ecological factor that impacts the growth and survival of aquatic organisms. The salinity of seawater in coastal and estuarine areas is often subject to dynamic changes because of seasonal rainfall and continental runoff. Thus, the current study investigated the effects of sudden changes in salinity on the survival rate and osmotic pressure regulation mechanisms of bottom-sowing seedlings of the economically important ark shell,Scapharca subcrenata. By simulating the sudden changes that occur in seawater salinity after rainstorms, the results showed that the osmotic pressure of the hemolymph and Na+, K+, Ca2+, and Cl(-)concentrations first decreased and then increased. When the salinity decreased from 30 to 14 parts per thousand, hemoglobin, soluble total protein, taurine, and total free amino acid gradually increased; maximum levels of hemoglobin, soluble total protein, and taurine occurred once the salinity increased to 22 parts per thousand at 96 h. After 96 h, the total free amino acid content increased until 144 h. The reactive oxygen species (ROS) content and total antioxidant capacity (T-AOC) peaked at 96 h, whereas the expression levels of Mn-superoxide dismutase (MnSOD) and catalase (CAT) increased earlier, indicating that, with continuous ROS generation, antioxidant defense mechanisms were activated to avoid oxidative damage. Expression levels of cathepsin C (CTSC), cathepsin D (CTSD), heat shock protein 20 (HSP20), and heat shock protein 70 (HSP70) were significantly higher than in the control group at 48 h (salinity level 14 parts per thousand); the expression levels ofHSP20, heat shock protein 90 (HSP90),MnSOD, and glutathione peroxidase (GP(x)) remained high, indicating that they were still required for osmotic pressure regulation to maintain the dynamic balance between the generation and removal of ROS as the salinity level increased. These results not only add to our basic understanding of the aquatic ecology ofS. subcrenata, but also provide a theoretical ground for improving the survival rate of bottom-sowing, propagation, and release ofS. subcrenataseedlings.
KeywordScapharca subcrenata salinity osmotic pressure reactive oxygen species antioxidant defense heat shock proteins cathepsin
DOI10.3389/fphys.2020.00884
Indexed BySCI
Language英语
Funding ProjectChina Postdoctoral Science Foundation[2019M650153] ; Modern Agro-industry Technology Research System in Shandong Province[SDAIT-14-04] ; Outstanding Postdoctoral Scholarship from the State Key Laboratory of Marine Environmental Science at Xiamen University
WOS Research AreaPhysiology
WOS SubjectPhysiology
WOS IDWOS:000555800100001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168106
Collection实验海洋生物学重点实验室
Corresponding AuthorGao Xiaolong
Affiliation1.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Marine Biol Inst Shandong Prov, Qingdao, Peoples R China
4.Dalian Ocean Univ, Coll Marine Technol & Environm, Dalian, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang Mo,Li Li,Liu Ying,et al. Effects of Sudden Drop in Salinity on Osmotic Pressure Regulation and Antioxidant Defense Mechanism ofScapharca subcrenata[J]. FRONTIERS IN PHYSIOLOGY,2020,11:14.
APA Zhang Mo,Li Li,Liu Ying,&Gao Xiaolong.(2020).Effects of Sudden Drop in Salinity on Osmotic Pressure Regulation and Antioxidant Defense Mechanism ofScapharca subcrenata.FRONTIERS IN PHYSIOLOGY,11,14.
MLA Zhang Mo,et al."Effects of Sudden Drop in Salinity on Osmotic Pressure Regulation and Antioxidant Defense Mechanism ofScapharca subcrenata".FRONTIERS IN PHYSIOLOGY 11(2020):14.
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