Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus

doi:10.1016/j.aquaculture.2020.735493

IOCAS-IR > 实验海洋生物学重点实验室

	Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus
	Liang, Shaoshuai 1,2; Wang, Wenxiang 1,2,3; Wang, Lijuan1,2 ; Wu, Zhihao 1,2; Zou, Yuxia 1,2; Tan, Xungang1,2 ; Liu, Yan1,2 ; Peng, Zhuangzhuang 1,2; You, Feng 1,2
	2020-11-15
发表期刊	AQUACULTURE
ISSN	0044-8486
卷号	528 页码:11
摘要	Factor in the germ line alpha (a basic helix-loop-helix transcription factor, FIGLA) gene specifically expresses in female germ cells and is essential for ovarian differentiation and development in vertebrates. However, research on the roles in fish gonadal differentiation and development is limited. In this study, we cloned and characterized a figla gene from the olive flounder, Paralichthys olivaceus, an important marine cultured fish in China, Japan, and Korea. The full-length cDNA of the Po-figla is 1316 bp along with a 609 bp open reading frame. Real-time quantitative polymerase chain reaction analysis (qPCR) revealed that it was abundantly expressed in the ovary and female brain. During stages I-V of the ovary, the level of Po-figla mRNA reached the peak at stage II, and then gradually decreased, while Po-figla mRNA was slightly expressed along with the testicular development. Further, one color and two-color in situ hybridization results showed that the gene was mainly located in oocytes. Knocking down Po-figla in the ovary using RNAi led to its down-regulated expression and the reduction of oocyte number. In addition, qPCR results presented the differential expression patterns of Po-figla during gonadal differentiation period. In the exogenous 17 beta-estradiol treatment group (5 ppm), Po-figla expression levels constitutively increased in the differentiating and differentiated ovaries, while in the testosterone (5 ppm) treatment group, it was lower expressed in the testis during testicular differentiation period. Our findings provide evidence that Po-figla is involved in P. olivaceus ovarian differentiation and development, especially might have roles in the proliferation of fish oocytes. These findings provide the basic data for fish reproduction and gonadal development regulation in aquaculture.
关键词	Figla Female-predominant expression RNAi Oocyte Olive flounder Paralichthys olivaceus
DOI	10.1016/j.aquaculture.2020.735493
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2018YFD0900202] ; National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31872558] ; National Natural Science Foundation of China[31702337] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000553682600010
出版者	ELSEVIER
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/168097
专题	实验海洋生物学重点实验室
通讯作者	You, Feng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Liang, Shaoshuai,Wang, Wenxiang,Wang, Lijuan,et al. Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus[J]. AQUACULTURE,2020,528:11.
APA	Liang, Shaoshuai.,Wang, Wenxiang.,Wang, Lijuan.,Wu, Zhihao.,Zou, Yuxia.,...&You, Feng.(2020).Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus.AQUACULTURE,528,11.
MLA	Liang, Shaoshuai,et al."Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus".AQUACULTURE 528(2020):11.

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