Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus | |
Liang, Shaoshuai1,2; Wang, Wenxiang1,2,3; Wang, Lijuan1,2 ; Wu, Zhihao1,2; Zou, Yuxia1,2; Tan, Xungang1,2; Liu, Yan1,2; Peng, Zhuangzhuang1,2; You, Feng1,2
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| 2020-11-15 | |
| Source Publication | AQUACULTURE
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| ISSN | 0044-8486 |
| Volume | 528Pages:11 |
| Abstract | Factor in the germ line alpha (a basic helix-loop-helix transcription factor, FIGLA) gene specifically expresses in female germ cells and is essential for ovarian differentiation and development in vertebrates. However, research on the roles in fish gonadal differentiation and development is limited. In this study, we cloned and characterized a figla gene from the olive flounder, Paralichthys olivaceus, an important marine cultured fish in China, Japan, and Korea. The full-length cDNA of the Po-figla is 1316 bp along with a 609 bp open reading frame. Real-time quantitative polymerase chain reaction analysis (qPCR) revealed that it was abundantly expressed in the ovary and female brain. During stages I-V of the ovary, the level of Po-figla mRNA reached the peak at stage II, and then gradually decreased, while Po-figla mRNA was slightly expressed along with the testicular development. Further, one color and two-color in situ hybridization results showed that the gene was mainly located in oocytes. Knocking down Po-figla in the ovary using RNAi led to its down-regulated expression and the reduction of oocyte number. In addition, qPCR results presented the differential expression patterns of Po-figla during gonadal differentiation period. In the exogenous 17 beta-estradiol treatment group (5 ppm), Po-figla expression levels constitutively increased in the differentiating and differentiated ovaries, while in the testosterone (5 ppm) treatment group, it was lower expressed in the testis during testicular differentiation period. Our findings provide evidence that Po-figla is involved in P. olivaceus ovarian differentiation and development, especially might have roles in the proliferation of fish oocytes. These findings provide the basic data for fish reproduction and gonadal development regulation in aquaculture. |
| Keyword | Figla Female-predominant expression RNAi Oocyte Olive flounder Paralichthys olivaceus |
| DOI | 10.1016/j.aquaculture.2020.735493 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2018YFD0900202] ; National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31872558] ; National Natural Science Foundation of China[31702337] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470] |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000553682600010 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/168097 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | You, Feng |
| Affiliation | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing 10049, Peoples R China |
| First Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Liang, Shaoshuai,Wang, Wenxiang,Wang, Lijuan,et al. Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus[J]. AQUACULTURE,2020,528:11. |
| APA | Liang, Shaoshuai.,Wang, Wenxiang.,Wang, Lijuan.,Wu, Zhihao.,Zou, Yuxia.,...&You, Feng.(2020).Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus.AQUACULTURE,528,11. |
| MLA | Liang, Shaoshuai,et al."Figla gene roles in the proliferation of oocytes in the olive flounder Paralichthys olivaceus".AQUACULTURE 528(2020):11. |
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| Figla gene roles in （2465KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View | |
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