Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Insight into AMPK regulation mechanism in vivo and in vitro: Responses to low temperatures in the olive flounder Paralichthys olivaceus | |
Nie, Miaomiao1,2,3; Lu, Yunliang4; Zou, Congcong1,2,3 ; Wang, Lijuan1,2; Zhang, Peijun1,2; You, Feng1,2
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| 2020-07-01 | |
| 发表期刊 | JOURNAL OF THERMAL BIOLOGY
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| ISSN | 0306-4565 |
| 卷号 | 91页码:9 |
| 通讯作者 | You, Feng(youfeng@qdio.ac.cn) |
| 摘要 | The olive flounder, Paralichthys olivaceus, is a commercially important maricultured fish in China, Japan, and Korea. Low winter temperatures influence its survival and growth and affect the output of the aquaculture industry. Energy metabolism is essential for fish survival, and the central energy-regulating factor - 5'-AMP-activated protein kinase (AMPK) - plays an important role in responses to cold stress. However, the mechanism of AMPK pathway regulation in fish coping with cold stress remains poorly understood. In the present study, the expression of AMPK and its upstream (LKB1 and CaMKK beta) and downstream genes (SITR1, FOXO1A, and TFAM) in the brain, muscle, and heart was analyzed while the flounder was under cold stress (0.2 +/- 0.2 degrees C). The results showed that low temperatures activated LKB1, CaMKK beta, and AMPK genes in the brain, and the activated AMPK induced expression of SITR1, FOXO1A, and TFAM. In the muscle tissue, the expression patterns of these genes presented a trend of initially decreasing and then increasing, and there was a delay in the response to low temperatures. At the cellular level, comparative analysis of the effects of the activator 5-aminoimidazole-4-carboxamide1-beta-D-ribofuranoside (AICAR) and inhibitor compound C of the AMPK pathway demonstrated that cold stress was similar to AICAR, which activated the AMPK pathway with hysteresis. Thus, the regulation mechanism of AMPK under cold stress was preliminarily analyzed. In general, AMPK was involved not only in responses to low temperatures but also in energy regulation under cold stress. |
| 关键词 | Paralichthys olivaceus AMPK pathway Cold stress Activator Inhibitor |
| DOI | 10.1016/j.jtherbio.2020.102640 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[31602143] ; Project of Zhejiang Province of China[2017C04003] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Zoology |
| WOS类目 | Biology ; Zoology |
| WOS记录号 | WOS:000552838300004 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/168091 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | You, Feng |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao, Peoples R China |
| 第一作者单位 | 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Nie, Miaomiao,Lu, Yunliang,Zou, Congcong,et al. Insight into AMPK regulation mechanism in vivo and in vitro: Responses to low temperatures in the olive flounder Paralichthys olivaceus[J]. JOURNAL OF THERMAL BIOLOGY,2020,91:9. |
| APA | Nie, Miaomiao,Lu, Yunliang,Zou, Congcong,Wang, Lijuan,Zhang, Peijun,&You, Feng.(2020).Insight into AMPK regulation mechanism in vivo and in vitro: Responses to low temperatures in the olive flounder Paralichthys olivaceus.JOURNAL OF THERMAL BIOLOGY,91,9. |
| MLA | Nie, Miaomiao,et al."Insight into AMPK regulation mechanism in vivo and in vitro: Responses to low temperatures in the olive flounder Paralichthys olivaceus".JOURNAL OF THERMAL BIOLOGY 91(2020):9. |
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