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Insight into AMPK regulation mechanism in vivo and in vitro: Responses to low temperatures in the olive flounder Paralichthys olivaceus
Nie, Miaomiao1,2,3; Lu, Yunliang4; Zou, Congcong1,2,3; Wang, Lijuan1,2; Zhang, Peijun1,2; You, Feng1,2
2020-07-01
Source PublicationJOURNAL OF THERMAL BIOLOGY
ISSN0306-4565
Volume91Pages:9
Corresponding AuthorYou, Feng(youfeng@qdio.ac.cn)
AbstractThe olive flounder, Paralichthys olivaceus, is a commercially important maricultured fish in China, Japan, and Korea. Low winter temperatures influence its survival and growth and affect the output of the aquaculture industry. Energy metabolism is essential for fish survival, and the central energy-regulating factor - 5'-AMP-activated protein kinase (AMPK) - plays an important role in responses to cold stress. However, the mechanism of AMPK pathway regulation in fish coping with cold stress remains poorly understood. In the present study, the expression of AMPK and its upstream (LKB1 and CaMKK beta) and downstream genes (SITR1, FOXO1A, and TFAM) in the brain, muscle, and heart was analyzed while the flounder was under cold stress (0.2 +/- 0.2 degrees C). The results showed that low temperatures activated LKB1, CaMKK beta, and AMPK genes in the brain, and the activated AMPK induced expression of SITR1, FOXO1A, and TFAM. In the muscle tissue, the expression patterns of these genes presented a trend of initially decreasing and then increasing, and there was a delay in the response to low temperatures. At the cellular level, comparative analysis of the effects of the activator 5-aminoimidazole-4-carboxamide1-beta-D-ribofuranoside (AICAR) and inhibitor compound C of the AMPK pathway demonstrated that cold stress was similar to AICAR, which activated the AMPK pathway with hysteresis. Thus, the regulation mechanism of AMPK under cold stress was preliminarily analyzed. In general, AMPK was involved not only in responses to low temperatures but also in energy regulation under cold stress.
KeywordParalichthys olivaceus AMPK pathway Cold stress Activator Inhibitor
DOI10.1016/j.jtherbio.2020.102640
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31602143] ; Project of Zhejiang Province of China[2017C04003] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS Research AreaLife Sciences & Biomedicine - Other Topics ; Zoology
WOS SubjectBiology ; Zoology
WOS IDWOS:000552838300004
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168091
Collection实验海洋生物学重点实验室
Corresponding AuthorYou, Feng
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Nie, Miaomiao,Lu, Yunliang,Zou, Congcong,et al. Insight into AMPK regulation mechanism in vivo and in vitro: Responses to low temperatures in the olive flounder Paralichthys olivaceus[J]. JOURNAL OF THERMAL BIOLOGY,2020,91:9.
APA Nie, Miaomiao,Lu, Yunliang,Zou, Congcong,Wang, Lijuan,Zhang, Peijun,&You, Feng.(2020).Insight into AMPK regulation mechanism in vivo and in vitro: Responses to low temperatures in the olive flounder Paralichthys olivaceus.JOURNAL OF THERMAL BIOLOGY,91,9.
MLA Nie, Miaomiao,et al."Insight into AMPK regulation mechanism in vivo and in vitro: Responses to low temperatures in the olive flounder Paralichthys olivaceus".JOURNAL OF THERMAL BIOLOGY 91(2020):9.
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