IOCAS-IR  > 海洋生态与环境科学重点实验室
Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons
Li Mengna1,2,5; Chen Hao1,2,3,4; Wang Minxiao1,2,3,4; Zhong Zhaoshan1,2,5; Zhou Li1,2,3,4; Li Chaolun1,2,3,4,5
2020-07-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume38Issue:4Pages:1292-1303
Corresponding AuthorLi Chaolun(lcl@qdio.ac.cn)
AbstractDeep-sea mussels of the subfamily Bathymodiolinae are common and numerically dominant species widely distributed in cold seeps and hydrothermal vents. During long-time evolution, deep-sea mussels have evolved to be well adapted to the local environment of cold seeps and hydrothermal vents by various ways, especially by establishing endosymbiosis with chemotrophic bacteria. However, biological processes underlying the establishment and maintenance of symbiosis between host mussels and symbionts are largely unclear. In the present study,Gigantidas platifronsgenes possibly involved in the symbiosis with methane oxidation symbionts were identified and characterized by Lipopolysaccharide (LPS) pull-down andin situhybridization. Five immune related proteins including Toll-like receptor 2 (TLR2), integrin, vacuolar sorting protein (VSP), matrix metalloproteinase 1 (MMP1), and leucine-rich repeat (LRR-1) were identified by LPS pull-down assay. These five proteins were all conserved in either molecular sequences or functional domains and known to be key molecules in host immune recognition, phagocytosis, and lysosome-mediated digestion. Furthermore,in situhybridization of LRR-1, TLR2 and VSP genes was conducted to investigate their expression patterns in gill tissues ofG. platifrons. Consequently, LRR-1, TLR2, and VSP genes were found expressed exclusively in the bacteriocytes ofG. platifrons. Therefore, it was suggested that TLR2, integrin, VSP, MMP1, and LRR-1 might be crucial molecules in the symbiosis betweenG. platifronsand methane oxidation bacteria by participating in symbiosis-related immune processes.
KeywordGigantidas platifrons endosymbiosis innate immunity pull-down assay immune recognition methane oxidation bacteria
DOI10.1007/s00343-020-0040-7
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000551529700026
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/168042
Collection海洋生态与环境科学重点实验室
深海极端环境与生命过程研究中心
Corresponding AuthorLi Chaolun
Affiliation1.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci CODR & KLMEES, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li Mengna,Chen Hao,Wang Minxiao,et al. Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(4):1292-1303.
APA Li Mengna,Chen Hao,Wang Minxiao,Zhong Zhaoshan,Zhou Li,&Li Chaolun.(2020).Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(4),1292-1303.
MLA Li Mengna,et al."Identification and characterization of endosymbiosis-related immune genes in deep-sea musselsGigantidas platifrons".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.4(2020):1292-1303.
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