Effect of Fucoxanthin Administration on Thyroid Gland Injury Induced by Cadmium in Mice

doi:10.1007/s12011-020-02291-9

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	Effect of Fucoxanthin Administration on Thyroid Gland Injury Induced by Cadmium in Mice
	Yang, Haoyue1,2 ; Xing, Ronge1,2 ; Liu, Song 1,2; Li, Pengcheng1,2
	2020-07-21
发表期刊	BIOLOGICAL TRACE ELEMENT RESEARCH
ISSN	0163-4984
页码	8
摘要	The intervention effect of fucoxanthin, which is reportedly a powerful antioxidant, on cadmium-induced thyroid damage in mice was evaluated. Animals (N= 120) were divided into control group (given pure water,N= 20) and CdCl2-exposed group (given CdCl(2)orally at a dose of 30 mg/kg body weight (bw)/day for 30 days,N= 100). Besides, the CdCl2-exposed group was divided into the following 5 groups (N= 20) to evaluate the intervention effect of fucoxanthin: (1) negative control group (NCG; animals were supplied with pure water); (2) positive control group (PCG; animals were supplied with 50 mg/kg bw/day thyroid tablets. Thyroid tablets are made from the thyroid glands of pigs, cattle, sheep and other food animals (the main components of T4). But because they are extracts of the thyroid glands, they may contain a small amount of T3. The specific amount of T3 is unknown.); (3) low fucoxanthin concentration group (F1; animals were supplied with 10 mg/kg bw/day fucoxanthin); (4) medium fucoxanthin concentration group (F2; animals were supplied with 25 mg/kg bw/day fucoxanthin); (5) high fucoxanthin concentration groups (F3; animals were supplied with 50 mg/kg bw/day fucoxanthin). A 14-day treatment was conducted for these animals. The levels of T4, T3, MDA, ascorbate peroxidase (APX) and catalase were measured, and the expression levels ofBax,Bcl-2,ERK1,ERK2,MEK1,eIf2 alpha,p-eIf2 alpha,GRP78andGRP94genes were determined using real-time reverse transcriptase-polymerase chain reactions (RT-PCR). In addition, tissue histopathology and ultrastructure were recorded and analysed. We found that the injection of cadmium chloride (CdCl2) decreased serum T4 and T3 levels to 27.10 ng/ml and 837.74 pg/ml, respectively. In addition, CdCl(2)intoxication induced oxidative stress, structural abnormalities and apoptosis in thyroid follicles. Our results showed that the treatment of CaCl-exposed mice with 25-50 mg/kg bw/day fucoxanthin appreciably decreased oxidative stress and apoptosis induced by CdCl(2)and restored the microstructural and ultrastructural organisations of the thyroid gland towards normalcy. Compared with the negative control group, fucoxanthin treatment groups showed significant increase in T4 and T3 levels (52.17 ng/ml and 1669.18 ng/ml, respectively;P< 0.05), relieved oxidative stress by decreasing malondialdehyde level and increasing catalase and APX levels and increased apoptosis inhibition through inhibiting the ERK1/2 pathway and preventing endoplasmic reticulum stress in thyroid follicular epithelial cells. Herein, our study provides evidence of the protective effects of fucoxanthin supplementation against thyroid damage and suggests therapeutic potential of this pigment in cases of Cd intoxication and hypothyroidism.
关键词	Fucoxanthin Cadmium Thyrotoxicity Impaired thyroid function Anti-hypothyroidism
DOI	10.1007/s12011-020-02291-9
收录类别	SCI
语种	英语
资助项目	Key Research and Development Program of Shandong Province[2019GHY112015] ; Key Research and Development Program of Shandong Province[2017YYSP018] ; Qingdao 2016 marine economic innovation and development demonstration city project plan
WOS研究方向	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS类目	Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS记录号	WOS:000550594700001
出版者	HUMANA PRESS INC
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/168027
专题	实验海洋生物学重点实验室
通讯作者	Xing, Ronge
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Yang, Haoyue,Xing, Ronge,Liu, Song,et al. Effect of Fucoxanthin Administration on Thyroid Gland Injury Induced by Cadmium in Mice[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2020:8.
APA	Yang, Haoyue,Xing, Ronge,Liu, Song,&Li, Pengcheng.(2020).Effect of Fucoxanthin Administration on Thyroid Gland Injury Induced by Cadmium in Mice.BIOLOGICAL TRACE ELEMENT RESEARCH,8.
MLA	Yang, Haoyue,et al."Effect of Fucoxanthin Administration on Thyroid Gland Injury Induced by Cadmium in Mice".BIOLOGICAL TRACE ELEMENT RESEARCH (2020):8.

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