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Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273
Wu, Z.1; Zheng, R.2; Zhang, J.2; Wu, S.1
2020-07-16
Source PublicationJOURNAL OF APPLIED MICROBIOLOGY
ISSN1364-5072
Pages13
AbstractAims Relatively, few anti-biofilm polysaccharides againstPseudomonas aeruginosawere done to investigate the underlying molecular mechanism. Exopolysaccharide EPS273 can clearly reduce biofilm formation and infection ofP. aeruginosa. This study aims to investigate its anti-biofilm and anti-infection mechanism on transcriptional level. Methods and Results Herein, we used an RNA-Seq transcriptomic approach to investigate the underlying anti-biofilm and anti-infection mechanism of EPS273. The expression levels of a large number of genes were changed afterP. aeruginosaPAO1 was treated with EPS273. Especially, the genes related to biofilm formation, such as gene involved in production of extracellular matrix and virulence factor, genes involved in flagella and cell motility and genes involved in iron acquisition. Notably, the expression levels of genes involved in regulatory and signal transduction were markedly downregulated, such as two-component system PhoP-PhoQ and quorum sensing (QS) system LasI/LasR and RhlI/RhlR. Furthermore, when genesphoPandphoQwere disrupted, respectively, the reduction of biofilm formation and cell motility in mutant ophoPor ophoQwas also detected. Conclusion EPS273 may exert its anti-biofilm and anti-infection function by downregulating gene expression of two-component system PhoP-PhoQ and QS systems LasI/LasR and RhlI/RhlR ofP. aeruginosa, which further regulated expression of genes involved in biofilm formation. Significance and Impact of the Study Our data will expand understanding of anti-biofilm mechanisms of polysaccharides on transcriptomic level.
Keywordbiofilm polysaccharide Pseudomonas aeruginosa transcriptomic virulence factor
DOI10.1111/jam.14764
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31600035]
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000548698500001
PublisherWILEY
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/167943
Collection实验海洋生物学重点实验室
Corresponding AuthorWu, S.
Affiliation1.Qingdao Univ, Coll Life Sci, Qingdao, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Wu, Z.,Zheng, R.,Zhang, J.,et al. Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273[J]. JOURNAL OF APPLIED MICROBIOLOGY,2020:13.
APA Wu, Z.,Zheng, R.,Zhang, J.,&Wu, S..(2020).Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273.JOURNAL OF APPLIED MICROBIOLOGY,13.
MLA Wu, Z.,et al."Transcriptional profiling ofPseudomonas aeruginosaPAO1 in response to anti-biofilm and anti-infection agent exopolysaccharide EPS273".JOURNAL OF APPLIED MICROBIOLOGY (2020):13.
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