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Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles
Zeng, Yan1,2,3; Qi, Peng1,2,3; Zhang, Dun1,2,3
2020-07-14
Source PublicationANALYTICAL METHODS
ISSN1759-9660
Volume12Issue:26Pages:3404-3410
Corresponding AuthorQi, Peng() ; Zhang, Dun(zhangdun@qdio.ac.cn)
AbstractThe level ofd-amino acids (DAAs) is significantly related to bacterial contamination in health and disease, food science and nutrition, and industrial applications. Most sensing methods for the detection of DAAs need expensive equipment, and skilled operation experience, making the test kit for DAA analysis much more complex and challenging. Toward this end, we exploited a label-free DAA test kit based on 1,4-benzenediboronic acid (BDBA)-induced gold nanoparticles (AuNPs) aggregations. DAAs were first catalyzed by their specific catalase (DAAO,d-amino acid oxidase) and oxidized to produce H2O2. Then, the produced H(2)O(2)inhibited the citrate-capped AuNPs aggregation under BDBA, while the unreacted BDBA could lead to AuNPs aggregation. As a result, the UV/vis absorption spectra and optical photographs of the AuNPs solution are changed in the presence of different DAA target amounts. This method not only can be used for qualitative and semi-quantitative analysis with a naked-eye readout, but also it can quantify DAAs in aqueous solutions with high sensitivity and specificity. Furthermore, the DAAs test kit provided an effective and selective quantification of DAAs with excellent precision and accuracy in bacterial samples. We believe this DAA test kit provides the potential to be further developed for DAA detection for satisfying both lab and practical needs in different fields.
DOI10.1039/d0ay00800a
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41876101] ; National Natural Science Foundation of China[51771180] ; National Natural Science Foundation of China[41906037] ; Young Elite Scientists Sponsorship Program by CAST[20180276]
WOS Research AreaChemistry ; Food Science & Technology ; Spectroscopy
WOS SubjectChemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS IDWOS:000546454400012
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167817
Collection海洋环境腐蚀与与生物污损重点实验室
Corresponding AuthorQi, Peng; Zhang, Dun
Affiliation1.Chinese Acad Sci, Key Lab Marine Environm Corros & Biofouling, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zeng, Yan,Qi, Peng,Zhang, Dun. Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles[J]. ANALYTICAL METHODS,2020,12(26):3404-3410.
APA Zeng, Yan,Qi, Peng,&Zhang, Dun.(2020).Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles.ANALYTICAL METHODS,12(26),3404-3410.
MLA Zeng, Yan,et al."Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles".ANALYTICAL METHODS 12.26(2020):3404-3410.
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