Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles
Zeng, Yan1,2,3; Qi, Peng1,2,3; Zhang, Dun1,2,3
2020-07-14
发表期刊ANALYTICAL METHODS
ISSN1759-9660
卷号12期号:26页码:3404-3410
通讯作者Qi, Peng() ; Zhang, Dun(zhangdun@qdio.ac.cn)
摘要The level ofd-amino acids (DAAs) is significantly related to bacterial contamination in health and disease, food science and nutrition, and industrial applications. Most sensing methods for the detection of DAAs need expensive equipment, and skilled operation experience, making the test kit for DAA analysis much more complex and challenging. Toward this end, we exploited a label-free DAA test kit based on 1,4-benzenediboronic acid (BDBA)-induced gold nanoparticles (AuNPs) aggregations. DAAs were first catalyzed by their specific catalase (DAAO,d-amino acid oxidase) and oxidized to produce H2O2. Then, the produced H(2)O(2)inhibited the citrate-capped AuNPs aggregation under BDBA, while the unreacted BDBA could lead to AuNPs aggregation. As a result, the UV/vis absorption spectra and optical photographs of the AuNPs solution are changed in the presence of different DAA target amounts. This method not only can be used for qualitative and semi-quantitative analysis with a naked-eye readout, but also it can quantify DAAs in aqueous solutions with high sensitivity and specificity. Furthermore, the DAAs test kit provided an effective and selective quantification of DAAs with excellent precision and accuracy in bacterial samples. We believe this DAA test kit provides the potential to be further developed for DAA detection for satisfying both lab and practical needs in different fields.
DOI10.1039/d0ay00800a
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[41876101] ; National Natural Science Foundation of China[51771180] ; National Natural Science Foundation of China[41906037] ; Young Elite Scientists Sponsorship Program by CAST[20180276]
WOS研究方向Chemistry ; Food Science & Technology ; Spectroscopy
WOS类目Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS记录号WOS:000546454400012
出版者ROYAL SOC CHEMISTRY
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/167817
专题海洋环境腐蚀与生物污损重点实验室
通讯作者Qi, Peng; Zhang, Dun
作者单位1.Chinese Acad Sci, Key Lab Marine Environm Corros & Biofouling, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
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GB/T 7714
Zeng, Yan,Qi, Peng,Zhang, Dun. Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles[J]. ANALYTICAL METHODS,2020,12(26):3404-3410.
APA Zeng, Yan,Qi, Peng,&Zhang, Dun.(2020).Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles.ANALYTICAL METHODS,12(26),3404-3410.
MLA Zeng, Yan,et al."Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles".ANALYTICAL METHODS 12.26(2020):3404-3410.
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