Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
| Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles | |
Zeng, Yan1,2,3 ; Qi, Peng1,2,3; Zhang, Dun1,2,3
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| 2020-07-14 | |
| Source Publication | ANALYTICAL METHODS
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| ISSN | 1759-9660 |
| Volume | 12Issue:26Pages:3404-3410 |
| Corresponding Author | Qi, Peng() ; Zhang, Dun(zhangdun@qdio.ac.cn) |
| Abstract | The level ofd-amino acids (DAAs) is significantly related to bacterial contamination in health and disease, food science and nutrition, and industrial applications. Most sensing methods for the detection of DAAs need expensive equipment, and skilled operation experience, making the test kit for DAA analysis much more complex and challenging. Toward this end, we exploited a label-free DAA test kit based on 1,4-benzenediboronic acid (BDBA)-induced gold nanoparticles (AuNPs) aggregations. DAAs were first catalyzed by their specific catalase (DAAO,d-amino acid oxidase) and oxidized to produce H2O2. Then, the produced H(2)O(2)inhibited the citrate-capped AuNPs aggregation under BDBA, while the unreacted BDBA could lead to AuNPs aggregation. As a result, the UV/vis absorption spectra and optical photographs of the AuNPs solution are changed in the presence of different DAA target amounts. This method not only can be used for qualitative and semi-quantitative analysis with a naked-eye readout, but also it can quantify DAAs in aqueous solutions with high sensitivity and specificity. Furthermore, the DAAs test kit provided an effective and selective quantification of DAAs with excellent precision and accuracy in bacterial samples. We believe this DAA test kit provides the potential to be further developed for DAA detection for satisfying both lab and practical needs in different fields. |
| DOI | 10.1039/d0ay00800a |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[41876101] ; National Natural Science Foundation of China[51771180] ; National Natural Science Foundation of China[41906037] ; Young Elite Scientists Sponsorship Program by CAST[20180276] |
| WOS Research Area | Chemistry ; Food Science & Technology ; Spectroscopy |
| WOS Subject | Chemistry, Analytical ; Food Science & Technology ; Spectroscopy |
| WOS ID | WOS:000546454400012 |
| Publisher | ROYAL SOC CHEMISTRY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167817 |
| Collection | 海洋环境腐蚀与与生物污损重点实验室 |
| Corresponding Author | Qi, Peng; Zhang, Dun |
| Affiliation | 1.Chinese Acad Sci, Key Lab Marine Environm Corros & Biofouling, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zeng, Yan,Qi, Peng,Zhang, Dun. Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles[J]. ANALYTICAL METHODS,2020,12(26):3404-3410. |
| APA | Zeng, Yan,Qi, Peng,&Zhang, Dun.(2020).Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles.ANALYTICAL METHODS,12(26),3404-3410. |
| MLA | Zeng, Yan,et al."Label-free test kit ford-amino acid analysis by 1,4-benzenediboronic-acid-induced aggregation of gold nanoparticles".ANALYTICAL METHODS 12.26(2020):3404-3410. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Label-free test kit （656KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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