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Functional characterization of retinoid X receptor with an emphasis on the mediation of organotin poisoning in the Pacific oyster (Crassostrea gigas)
Huang, Wen1,2; Wu, Qian2; Xu, Fei1,3,4; Li, Li1,3,4; Li, Juan1; Que, Huayong1,3,4; Zhang, Guofan1,3,4
2020-08-30
Source PublicationGENE
ISSN0378-1119
Volume753Pages:7
AbstractMarine mollusks suffer harmful effects due to environmental organotin compounds such as tributyltin (TBT) and triphenyltin (TPT). It is known that gastropod imposex caused by organotins is mediated by a key nuclear receptor, retinoid X receptor (RXR). The organotin-mediated toxic effects on oysters grown in seawater include a thicker shell, incomplete growth, disrupted development and a high rate of mortality. However, few studies have been conducted to determine the role of RXR in the toxic effects of organotins on bivalves. Here, we cloned an RXR homolog (CgRXR) from the Pacific oyster (Crassostrea gigas) and characterized its molecular function. Expression of the CgRXR RNA transcripts was assessed in whole developmental stages and tissues, with the highest expression detected in the blastula and mantle, respectively. The subcellular localization experiment confirmed that CgRXR protein was expressed in the nucleus exclusively as a nuclear receptor. Electrophoretic mobility shift assay indicated that CgRXR could bind to the DNA motifs DR0-DR5. The dual-luciferase reporter assay demonstrated that the transcriptional activity of CgRXR was activated by conserved ligands (9-cis retinoic acid and cis-4,7,10,13,16,19-docosahexanoic acid) and endocrine-disrupting chemicals (TBT and TPT). These results revealed the conserved gene function involved in protein localization, ligand binding and heterodimer formation with thyroid hormone receptor. However, the DNA binding properties of CgRXR differed from those of other invertebrate and vertebrate RXRs. CgRXR had the highest expression level in the blastula and mantle, and the disrupted development or shell malformation induced by organotins suggested a possible correlation of CgRXR with shell formation in bivalves. The results indicated the potential involvement of CgRXR in the toxic effects of organotins (TBT and TPT) through signaling pathway disruption. Functional characterization of CgRXR will help us better understand the endocrinology of bivalves.
KeywordNuclear receptor Retinoid X receptor Organotin Crassostrea gigas Bivalve
DOI10.1016/j.gene.2020.144780
Indexed BySCI
Language英语
Funding ProjectGuangxi Natural Science Foundation[2017GXNSFAA198196] ; Guangxi Natural Science Foundation[2016GXNSFBA380028] ; National Natural Science Foundation of China[41866006] ; National Natural Science Foundation of China[41776152] ; National Natural Science Foundation of China[31372515] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Science[COMS2019Q11] ; China Agriculture Research System[CARS-49]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000541914900003
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/167811
Collection实验海洋生物学重点实验室
Corresponding AuthorXu, Fei; Que, Huayong
Affiliation1.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Guangxi Univ, Sch Marine Sci, Nanning 530004, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Huang, Wen,Wu, Qian,Xu, Fei,et al. Functional characterization of retinoid X receptor with an emphasis on the mediation of organotin poisoning in the Pacific oyster (Crassostrea gigas)[J]. GENE,2020,753:7.
APA Huang, Wen.,Wu, Qian.,Xu, Fei.,Li, Li.,Li, Juan.,...&Zhang, Guofan.(2020).Functional characterization of retinoid X receptor with an emphasis on the mediation of organotin poisoning in the Pacific oyster (Crassostrea gigas).GENE,753,7.
MLA Huang, Wen,et al."Functional characterization of retinoid X receptor with an emphasis on the mediation of organotin poisoning in the Pacific oyster (Crassostrea gigas)".GENE 753(2020):7.
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