IOCAS-IR  > 实验海洋生物学重点实验室
Biosynthesis of a Phycocyanin Beta Subunit with Two Noncognate Chromophores in Escherichia coli
Chen, Huaxin1,2,3; Zheng, Caiyun4; Jiang, Peng1,2,3; Ji, Gengsheng4
2020-06-01
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Volume191Issue:2Pages:763-771
Corresponding AuthorChen, Huaxin(chhx@qdio.ac.cn)
AbstractRecombinant phycobiliprotein can be used as fluorescent label in immunofluorescence assay. In this study, pathway for phycocyanin beta subunit (CpcB) carrying noncognate chromophore phycoerythrobilin (PEB) and phycourobilin (PUB) was constructed in Escherichia coli. Lyase CpcS and CpcT could catalyze attachment of PEB to Cys84-CpcB and Cys155-CpcB, respectively. However, PEB was attached only to Cys84-CpcB when both CpcS and CpcT were present in E. coli. A dual plasmid expression system was used to control the expression of lyases and the attachment order of PEB to CpcB. The production of PEB-Cys155-CpcB was achieved by L-arabinose-induced expression of CpcS, CpcB, Ho1, and PebS, and then the attachment of PEB to Cys84-CpcB was achieved by IPTG-induced expression of CpcS. The doubly chromophorylated CpcB absorbed light maximally at 497.5 nm and 557.0 nm and fluoresced maximally at 507.5 nm and 566.5 nm. An amount of light energy absorbed by PUB-Cys155-CpcB is transferred to PEB-Cys84-CpcB in doubly chromophorylated CpcB, conferring a large stokes shift of 69 nm for this fluorescent protein. There are interactions between chromophores of CpcB which possibly together with the help of lyases lead to isomerization of PEB-Cys155-CpcB to PUB-Cys155-CpcB.
KeywordPhycobiliprotein Phycocyanin Lyase Fluorescence Chromophore E coli
DOI10.1007/s12010-019-03219-3
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS SubjectBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS IDWOS:000541559900021
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167805
Collection实验海洋生物学重点实验室
Corresponding AuthorChen, Huaxin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Jiangsu Univ Sci & Technol, Coll Biotechnol Sericultural Res Inst, Zhenjiang, Jiangsu, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Huaxin,Zheng, Caiyun,Jiang, Peng,et al. Biosynthesis of a Phycocyanin Beta Subunit with Two Noncognate Chromophores in Escherichia coli[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2020,191(2):763-771.
APA Chen, Huaxin,Zheng, Caiyun,Jiang, Peng,&Ji, Gengsheng.(2020).Biosynthesis of a Phycocyanin Beta Subunit with Two Noncognate Chromophores in Escherichia coli.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,191(2),763-771.
MLA Chen, Huaxin,et al."Biosynthesis of a Phycocyanin Beta Subunit with Two Noncognate Chromophores in Escherichia coli".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 191.2(2020):763-771.
Files in This Item:
File Name/Size DocType Version Access License
Biosynthesis of a Ph(536KB)期刊论文出版稿开放获取CC BY-NC-SAView
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chen, Huaxin]'s Articles
[Zheng, Caiyun]'s Articles
[Jiang, Peng]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chen, Huaxin]'s Articles
[Zheng, Caiyun]'s Articles
[Jiang, Peng]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chen, Huaxin]'s Articles
[Zheng, Caiyun]'s Articles
[Jiang, Peng]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Biosynthesis of a Phycocyanin Beta Subunit with Two Noncognate Chromophores in Escherichia coli.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.