Investigation of EscA as a chaperone for the Edwardsiella tarda type III secretion system putative translocon component EseC

doi:10.1099/mic.0.021865-0

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	Investigation of EscA as a chaperone for the Edwardsiella tarda type III secretion system putative translocon component EseC
	Wang, Bo 1,2; Mo, Zhao Lan 1; Mao, Yun Xiang 3; Zou, Yu Xia 1; Xiao, Peng 1,2; Li, Jie3 ; Yang, Jia Yin 1,2; Ye, Xu Hong 3; Leung, Ka Yin 4; Zhang, Pei Jun 1; Mo, ZL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
	2009-04-01
发表期刊	MICROBIOLOGY-SGM
ISSN	1350-0872
卷号	155 期号:Part 4 页码:1260-1271
文章类型	Article
摘要	Edwardsiella tarda is an important Gram-negative enteric pathogen affecting both animals and humans. It possesses a type III secretion system (T3SS) essential for pathogenesis. EseB, EseC and EseD have been shown to form a translocon complex after secretion, while EscC functions as a T3SS chaperone for EseB and EseD. In this paper we identify EscA, a protein required for accumulation and proper secretion of another translocon component, EseC. The escA gene is located upstream of eseC and the EscA protein has the characteristics of T3SS chaperones. Cell fractionation experiments indicated that EscA is located in the cytoplasm and on the cytoplasmic membrane. Mutation with in-frame deletion of escA greatly decreased the secretion of EseC, while complementation of escA restored the wild-type secretion phenotype. The stabilization and accumulation of EseC in the cytoplasm were also affected in the absence of EscA. Mutation of escA did not affect the transcription of eseC but reduced the accumulation level of EseC as measured by using an EseC-LacZ fusion protein in Ed. tarda. Co-purification and co-immunoprecipitation studies demonstrated a specific interaction between EscA and EseC. Further analysis showed that residues 31-137 of EseC are required for EseC-EscA interaction, Mutation of EseC residues 31-137 reduced the secretion and accumulation of EseC in Ed. tarda. Finally, infection experiments showed that mutations of EscA and residues 31-137 of EseC increased the LD(50) by approximately 10-fold in blue gourami fish. These results indicated that EscA functions as a specific chaperone for EseC and contributes to the virulence of Ed. tarda.; Edwardsiella tarda is an important Gram-negative enteric pathogen affecting both animals and humans. It possesses a type III secretion system (T3SS) essential for pathogenesis. EseB, EseC and EseD have been shown to form a translocon complex after secretion, while EscC functions as a T3SS chaperone for EseB and EseD. In this paper we identify EscA, a protein required for accumulation and proper secretion of another translocon component, EseC. The escA gene is located upstream of eseC and the EscA protein has the characteristics of T3SS chaperones. Cell fractionation experiments indicated that EscA is located in the cytoplasm and on the cytoplasmic membrane. Mutation with in-frame deletion of escA greatly decreased the secretion of EseC, while complementation of escA restored the wild-type secretion phenotype. The stabilization and accumulation of EseC in the cytoplasm were also affected in the absence of EscA. Mutation of escA did not affect the transcription of eseC but reduced the accumulation level of EseC as measured by using an EseC-LacZ fusion protein in Ed. tarda. Co-purification and co-immunoprecipitation studies demonstrated a specific interaction between EscA and EseC. Further analysis showed that residues 31-137 of EseC are required for EseC-EscA interaction, Mutation of EseC residues 31-137 reduced the secretion and accumulation of EseC in Ed. tarda. Finally, infection experiments showed that mutations of EscA and residues 31-137 of EseC increased the LD50 by approximately 10-fold in blue gourami fish. These results indicated that EscA functions as a specific chaperone for EseC and contributes to the virulence of Ed. tarda.
关键词	Enteropathogenic Escherichia-coli Salmonella Pathogenicity Island-2 Tetratricopeptide Repeats Pseudomonas-aeruginosa Virulence Determinants Shigella-flexneri Protein Espd Typhimurium Bacteria Gene
学科领域	Microbiology
DOI	10.1099/mic.0.021865-0
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000265321700026
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1677
专题	实验海洋生物学重点实验室
通讯作者	Mo, ZL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Ocean Univ China, Qingdao 266003, Peoples R China 4.Natl Univ Singapore, Dept Biol Sci, Fac Sci, Singapore 117543, Singapore
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Wang, Bo,Mo, Zhao Lan,Mao, Yun Xiang,et al. Investigation of EscA as a chaperone for the Edwardsiella tarda type III secretion system putative translocon component EseC[J]. MICROBIOLOGY-SGM,2009,155(Part 4):1260-1271.
APA	Wang, Bo.,Mo, Zhao Lan.,Mao, Yun Xiang.,Zou, Yu Xia.,Xiao, Peng.,...&Mo, ZL, Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China.(2009).Investigation of EscA as a chaperone for the Edwardsiella tarda type III secretion system putative translocon component EseC.MICROBIOLOGY-SGM,155(Part 4),1260-1271.
MLA	Wang, Bo,et al."Investigation of EscA as a chaperone for the Edwardsiella tarda type III secretion system putative translocon component EseC".MICROBIOLOGY-SGM 155.Part 4(2009):1260-1271.

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