Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Genome-wide analysis of the Saccharina japonica sulfotransferase genes and their transcriptional profiles during whole developmental periods and under abiotic stresses | |
Lu, Chang1,2,3; Shao, Zhanru1,2; Zhang, Pengyan2,4 ; Duan, Delin1,2,5
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| 2020-06-11 | |
| Source Publication | BMC PLANT BIOLOGY
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| ISSN | 1471-2229 |
| Volume | 20Issue:1Pages:20 |
| Corresponding Author | Shao, Zhanru(zrshao@qdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn) |
| Abstract | Background As a unique sulfated polysaccharide, fucoidan is an important component of cell wall in brown seaweeds. Its biochemical properties are determined by the positions and quantity of sulfate groups. Sulfotransferases (STs) catalyze the sulfation process, which transfer the sulfuryl groups to carbohydrate backbones and are crucial for fucoidan biosynthesis. Nevertheless, the structures and functions ofSTsin brown seaweeds are rarely investigated. Results There are a total of 44 STgenes identified from our genome and transcriptome analysis ofSaccharina japonica, which were located in the 17 scaffolds and 11 contigs. TheS. japonica STgenes have abundant introns and alternative splicing sites, and five tandem duplicated gene clusters were identified. Generally, theSTgenes could be classified into five groups (Group I similar to V) based on phylogenetic analysis. Accordingly, the ST proteins, which were encoded by genes within the same group, contained similar conserved motifs. Members of theS. japonica STgene family show various expression patterns in different tissues and developmental stages. Transcriptional profiles indicate that the transcriptional levels of more than half of theSTgenes are higher in kelp basal blades than in distal blades. Except forST5andST28,mostSTgenes are down-regulated with the kelp development stages. The expression levels of nineST geneswere detected by real-time quantitative PCR, which demonstrates that they responded to low salinity and drought stresses. Conclusions Various characteristics of theSTsallow the feasibilities ofS. japonicato synthesize fucoidans with different sulfate groups. This enables the kelp the potential to adapt to the costal environments and meet the needs ofS. japonica growth. |
| Keyword | Saccharina japonica Sulfotransferase Fucoidan Transcriptome Genome |
| DOI | 10.1186/s12870-020-02422-3 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[41806175] ; Marine Biology and Biotechnology Laboratory Youth Foundation[YQ2018NO06] ; Shandong Key Research and Development Program[2018GHY115023] ; Joint Research Project between China and Japan[2017YFE0130900] |
| WOS Research Area | Plant Sciences |
| WOS Subject | Plant Sciences |
| WOS ID | WOS:000542241100002 |
| Publisher | BMC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167790 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Shao, Zhanru; Duan, Delin |
| Affiliation | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Div Mariculture Ecol & Technol, Qingdao 266071, Peoples R China 5.Qingdao Bright Moon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Peoples R China |
| First Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Lu, Chang,Shao, Zhanru,Zhang, Pengyan,et al. Genome-wide analysis of the Saccharina japonica sulfotransferase genes and their transcriptional profiles during whole developmental periods and under abiotic stresses[J]. BMC PLANT BIOLOGY,2020,20(1):20. |
| APA | Lu, Chang,Shao, Zhanru,Zhang, Pengyan,&Duan, Delin.(2020).Genome-wide analysis of the Saccharina japonica sulfotransferase genes and their transcriptional profiles during whole developmental periods and under abiotic stresses.BMC PLANT BIOLOGY,20(1),20. |
| MLA | Lu, Chang,et al."Genome-wide analysis of the Saccharina japonica sulfotransferase genes and their transcriptional profiles during whole developmental periods and under abiotic stresses".BMC PLANT BIOLOGY 20.1(2020):20. |
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| Genome-wide analysis（3107KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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