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Genome-wide analysis of the Saccharina japonica sulfotransferase genes and their transcriptional profiles during whole developmental periods and under abiotic stresses
Lu, Chang1,2,3; Shao, Zhanru1,2; Zhang, Pengyan2,4; Duan, Delin1,2,5
2020-06-11
Source PublicationBMC PLANT BIOLOGY
ISSN1471-2229
Volume20Issue:1Pages:20
Corresponding AuthorShao, Zhanru(zrshao@qdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn)
AbstractBackground As a unique sulfated polysaccharide, fucoidan is an important component of cell wall in brown seaweeds. Its biochemical properties are determined by the positions and quantity of sulfate groups. Sulfotransferases (STs) catalyze the sulfation process, which transfer the sulfuryl groups to carbohydrate backbones and are crucial for fucoidan biosynthesis. Nevertheless, the structures and functions ofSTsin brown seaweeds are rarely investigated. Results There are a total of 44 STgenes identified from our genome and transcriptome analysis ofSaccharina japonica, which were located in the 17 scaffolds and 11 contigs. TheS. japonica STgenes have abundant introns and alternative splicing sites, and five tandem duplicated gene clusters were identified. Generally, theSTgenes could be classified into five groups (Group I similar to V) based on phylogenetic analysis. Accordingly, the ST proteins, which were encoded by genes within the same group, contained similar conserved motifs. Members of theS. japonica STgene family show various expression patterns in different tissues and developmental stages. Transcriptional profiles indicate that the transcriptional levels of more than half of theSTgenes are higher in kelp basal blades than in distal blades. Except forST5andST28,mostSTgenes are down-regulated with the kelp development stages. The expression levels of nineST geneswere detected by real-time quantitative PCR, which demonstrates that they responded to low salinity and drought stresses. Conclusions Various characteristics of theSTsallow the feasibilities ofS. japonicato synthesize fucoidans with different sulfate groups. This enables the kelp the potential to adapt to the costal environments and meet the needs ofS. japonica growth.
KeywordSaccharina japonica Sulfotransferase Fucoidan Transcriptome Genome
DOI10.1186/s12870-020-02422-3
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41806175] ; Marine Biology and Biotechnology Laboratory Youth Foundation[YQ2018NO06] ; Shandong Key Research and Development Program[2018GHY115023] ; Joint Research Project between China and Japan[2017YFE0130900]
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000542241100002
PublisherBMC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167790
Collection实验海洋生物学重点实验室
Corresponding AuthorShao, Zhanru; Duan, Delin
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Div Mariculture Ecol & Technol, Qingdao 266071, Peoples R China
5.Qingdao Bright Moon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Lu, Chang,Shao, Zhanru,Zhang, Pengyan,et al. Genome-wide analysis of the Saccharina japonica sulfotransferase genes and their transcriptional profiles during whole developmental periods and under abiotic stresses[J]. BMC PLANT BIOLOGY,2020,20(1):20.
APA Lu, Chang,Shao, Zhanru,Zhang, Pengyan,&Duan, Delin.(2020).Genome-wide analysis of the Saccharina japonica sulfotransferase genes and their transcriptional profiles during whole developmental periods and under abiotic stresses.BMC PLANT BIOLOGY,20(1),20.
MLA Lu, Chang,et al."Genome-wide analysis of the Saccharina japonica sulfotransferase genes and their transcriptional profiles during whole developmental periods and under abiotic stresses".BMC PLANT BIOLOGY 20.1(2020):20.
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