IOCAS-IR  > 海洋生态与环境科学重点实验室
High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea
Sun, Qing-Lei1,2; Zhang, Jian1,2,3; Wang, Min-Xiao3,4; Cao, Lei3; Du, Zeng-Feng3,5; Sun, Yuan-Yuan1,2; Liu, Shi-Qi6; Li, Chao-Lun3,4,7; Sun, Li1,2
2020-05-01
发表期刊MICROORGANISMS
卷号8期号:5页码:18
通讯作者Li, Chao-Lun(lcl@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
摘要In the Formosa cold seep of the South China Sea (SCS), large amounts of methane and sulfide hydrogen are released from the subseafloor. In this study, we systematically investigated the microbial communities in the seawater-sediment interface of Formosa cold seep using high-throughput sequencing techniques including amplicon sequencing based on next-generation sequencing and Pacbio amplicon sequencing platforms, and metagenomics. We found that Sulfurovum dominated the microbial communities in the sediment-seawater interface, including the seawater close to the seepage, the surface sediments, and the gills of the dominant animal inhabitant (Shinkaia crosnieri). A nearly complete 16S rRNA gene sequence of the dominant operational taxonomic units (OTUs) was obtained from the Pacbio sequencing platforms and classified as OTU-L1, which belonged to Sulfurovum. This OTU was potentially novel as it shared relatively low similarity percentages (<97%) of the gene sequence with its close phylogenetic species. Further, a draft genome of Sulfurovum was assembled using the binning technique based on metagenomic data. Genome analysis suggested that Sulfurovum sp. in this region may fix carbon by the reductive tricarboxylic acid (rTCA) pathway, obtain energy by oxidizing reduced sulfur through sulfur oxidizing (Sox) pathway, and utilize nitrate as electron acceptors. These results demonstrated that Sulfurovum probably plays an important role in the carbon, sulfur, and nitrogen cycles of the Formosa cold seep of the SCS. This study improves our understanding of the diversity, distribution, and function of sulfur-oxidizing bacteria in deep-sea cold seep.
关键词cold seep seawater-sediment interface sulfur-oxidizing bacteria Campylobacterota Sulfurovum
DOI10.3390/microorganisms8050687
收录类别SCI
语种英语
资助项目National Key Research and Development Project of China[2018YFC0310801] ; Chinese Academy of Sciences[NMST-KEXUE2017K01] ; National Natural Science Foundation of China[41806202] ; Taishan Scholar Program of Shandong Province ; Senior User Project of RV KEXUE[KEXUE2018G20] ; Senior User Project of RV KEXUE[KEXUE2019GZ02]
WOS研究方向Microbiology
WOS类目Microbiology
WOS记录号WOS:000540222300068
出版者MDPI
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/167775
专题海洋生态与环境科学重点实验室
海洋地质与环境重点实验室
深海极端环境与生命过程研究中心
通讯作者Li, Chao-Lun; Sun, Li
作者单位1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Deep Sea Res Ctr, Inst Oceanol, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China
6.Univ Amsterdam, Fac Sci, NL-1098 XH Amsterdam, Netherlands
7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋研究所;  海洋生态与环境科学重点实验室;  中国科学院海洋大科学研究中心
推荐引用方式
GB/T 7714
Sun, Qing-Lei,Zhang, Jian,Wang, Min-Xiao,et al. High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea[J]. MICROORGANISMS,2020,8(5):18.
APA Sun, Qing-Lei.,Zhang, Jian.,Wang, Min-Xiao.,Cao, Lei.,Du, Zeng-Feng.,...&Sun, Li.(2020).High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea.MICROORGANISMS,8(5),18.
MLA Sun, Qing-Lei,et al."High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea".MICROORGANISMS 8.5(2020):18.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Highly sensitive vis(1434KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Sun, Qing-Lei]的文章
[Zhang, Jian]的文章
[Wang, Min-Xiao]的文章
百度学术
百度学术中相似的文章
[Sun, Qing-Lei]的文章
[Zhang, Jian]的文章
[Wang, Min-Xiao]的文章
必应学术
必应学术中相似的文章
[Sun, Qing-Lei]的文章
[Zhang, Jian]的文章
[Wang, Min-Xiao]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Highly sensitive visual detection of nucleic acid based on a universal strand exchange amplification coupled with lateral flow assay strip.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。