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High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea
Sun, Qing-Lei1,2; Zhang, Jian1,2,3; Wang, Min-Xiao3,4; Cao, Lei3; Du, Zeng-Feng3,5; Sun, Yuan-Yuan1,2; Liu, Shi-Qi6; Li, Chao-Lun3,4,7; Sun, Li1,2
2020-05-01
Source PublicationMICROORGANISMS
Volume8Issue:5Pages:18
Corresponding AuthorLi, Chao-Lun(lcl@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
AbstractIn the Formosa cold seep of the South China Sea (SCS), large amounts of methane and sulfide hydrogen are released from the subseafloor. In this study, we systematically investigated the microbial communities in the seawater-sediment interface of Formosa cold seep using high-throughput sequencing techniques including amplicon sequencing based on next-generation sequencing and Pacbio amplicon sequencing platforms, and metagenomics. We found that Sulfurovum dominated the microbial communities in the sediment-seawater interface, including the seawater close to the seepage, the surface sediments, and the gills of the dominant animal inhabitant (Shinkaia crosnieri). A nearly complete 16S rRNA gene sequence of the dominant operational taxonomic units (OTUs) was obtained from the Pacbio sequencing platforms and classified as OTU-L1, which belonged to Sulfurovum. This OTU was potentially novel as it shared relatively low similarity percentages (<97%) of the gene sequence with its close phylogenetic species. Further, a draft genome of Sulfurovum was assembled using the binning technique based on metagenomic data. Genome analysis suggested that Sulfurovum sp. in this region may fix carbon by the reductive tricarboxylic acid (rTCA) pathway, obtain energy by oxidizing reduced sulfur through sulfur oxidizing (Sox) pathway, and utilize nitrate as electron acceptors. These results demonstrated that Sulfurovum probably plays an important role in the carbon, sulfur, and nitrogen cycles of the Formosa cold seep of the SCS. This study improves our understanding of the diversity, distribution, and function of sulfur-oxidizing bacteria in deep-sea cold seep.
Keywordcold seep seawater-sediment interface sulfur-oxidizing bacteria Campylobacterota Sulfurovum
DOI10.3390/microorganisms8050687
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Project of China[2018YFC0310801] ; Chinese Academy of Sciences[NMST-KEXUE2017K01] ; National Natural Science Foundation of China[41806202] ; Taishan Scholar Program of Shandong Province ; Senior User Project of RV KEXUE[KEXUE2018G20] ; Senior User Project of RV KEXUE[KEXUE2019GZ02]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000540222300068
PublisherMDPI
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167775
Collection海洋生态与环境科学重点实验室
海洋地质与环境重点实验室
深海极端环境与生命过程研究中心
Corresponding AuthorLi, Chao-Lun; Sun, Li
Affiliation1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Deep Sea Res Ctr, Inst Oceanol, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China
6.Univ Amsterdam, Fac Sci, NL-1098 XH Amsterdam, Netherlands
7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Key Laboratory of Marine Ecology & Environmental Sciences, CAS;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Qing-Lei,Zhang, Jian,Wang, Min-Xiao,et al. High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea[J]. MICROORGANISMS,2020,8(5):18.
APA Sun, Qing-Lei.,Zhang, Jian.,Wang, Min-Xiao.,Cao, Lei.,Du, Zeng-Feng.,...&Sun, Li.(2020).High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea.MICROORGANISMS,8(5),18.
MLA Sun, Qing-Lei,et al."High-Throughput Sequencing Reveals a Potentially Novel Sulfurovum Species Dominating the Microbial Communities of the Seawater-Sediment Interface of a Deep-Sea Cold Seep in South China Sea".MICROORGANISMS 8.5(2020):18.
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