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Transcriptome analysis reveals seven key immune pathways of Japanese flounder (Paralichthys olivaceus) involved in megalocytivirus infection
Wu, Qian1,2,3; Ning, Xianhui1,2; Jiang, Shuai1,2; Sun, Li1,2
2020-08-01
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号103页码:150-158
摘要Megalocytivirus is a serious viral pathogen to many farmed fish including Japanese flounder (Paralichthys olivaceus). In this study, in order to systematically identify host immune genes induced by megalocytivirus infection, we examined the transcription profiles of flounder infected by megalocytivirus for 2, 6, and 8 days. Compared with uninfected fish, virus-infected fish exhibited 1242 differentially expressed genes (DEGs), with 225, 275, and 877 DEGs occurring at 2, 6, and 8 days post infection, respectively. Of these DEGs, 728 were upregulated and 659 were downregulated. The majority of DEGs were time-specific and formed four distinct expression profiles well correlated with the time of infection. The DEGs were classified into diverse Gene Ontology (GO) functional terms and enriched in 27 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, approximately one third of which were related to immunity. Weighted co-expression network analysis (WGCNA) was used to identify 16 key immune DEGs belonging to seven immune pathways (RIG-I-like receptor signaling pathway, JAK-STAT signaling pathway, TLR signaling pathway, cytokine-cytokine receptor interaction, phagosome, apoptosis, and p53 signaling pathway). These pathways interacted extensively and formed complicated networks. This study provided a global picture of megalocytivirus-induced gene expression profiles of flounder at the transcriptome level and uncovered a set of key immune genes and pathways closely linked to megalocytivirus infection. These results provided a set of targets for future delineation of the key factors implicated in the anti-megalocytivirus immunity of flounder.
关键词Paralichthys olivaceus Megalocytivirus Transcriptome Immune defense
DOI10.1016/j.fsi.2020.05.011
收录类别SCI
语种英语
资助项目Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Natural Science Foundation of China[31730100] ; National Key Research & Development Project of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000540375500018
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.qdio.ac.cn/handle/337002/167733
专题实验海洋生物学重点实验室
通讯作者Sun, Li
作者单位1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
第一作者单位中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋大科学研究中心
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Wu, Qian,Ning, Xianhui,Jiang, Shuai,et al. Transcriptome analysis reveals seven key immune pathways of Japanese flounder (Paralichthys olivaceus) involved in megalocytivirus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2020,103:150-158.
APA Wu, Qian,Ning, Xianhui,Jiang, Shuai,&Sun, Li.(2020).Transcriptome analysis reveals seven key immune pathways of Japanese flounder (Paralichthys olivaceus) involved in megalocytivirus infection.FISH & SHELLFISH IMMUNOLOGY,103,150-158.
MLA Wu, Qian,et al."Transcriptome analysis reveals seven key immune pathways of Japanese flounder (Paralichthys olivaceus) involved in megalocytivirus infection".FISH & SHELLFISH IMMUNOLOGY 103(2020):150-158.
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