Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| A novel bacterial thiosulfate oxidation pathway provides a new clue about the formation of zero-valent sulfur in deep sea | |
Zhang, Jing1,2,3,4,5 ; Liu, Rui1,2,3,5; Xi, Shichuan2,4,5,6; Cai, Ruining1,2,3,4,5; Zhang, Xin2,5,6; Sun, Chaomin1,2,3,5
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| 2020-05-26 | |
| 发表期刊 | ISME JOURNAL
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| ISSN | 1751-7362 |
| 页码 | 14 |
| 通讯作者 | Sun, Chaomin(sunchaomin@qdio.ac.cn) |
| 摘要 | Zero-valent sulfur (ZVS) has been shown to be a major sulfur intermediate in the deep-sea cold seep of the South China Sea based on our previous work, however, the microbial contribution to the formation of ZVS in cold seep has remained unclear. Here, we describe a novel thiosulfate oxidation pathway discovered in the deep-sea cold seep bacteriumErythrobacter flavus21-3, which provides a new clue about the formation of ZVS. Electronic microscopy, energy-dispersive, and Raman spectra were used to confirm thatE. flavus21-3 effectively converts thiosulfate to ZVS. We next used a combined proteomic and genetic method to identify thiosulfate dehydrogenase (TsdA) and thiosulfohydrolase (SoxB) playing key roles in the conversion of thiosulfate to ZVS. Stoichiometric results of different sulfur intermediates further clarify the function of TsdA in converting thiosulfate to tetrathionate (-O3S-S-S-SO3-), SoxB in liberating sulfone from tetrathionate to form ZVS and sulfur dioxygenases (SdoA/SdoB) in oxidizing ZVS to sulfite under some conditions. Notably, homologs of TsdA, SoxB, and SdoA/SdoB widely exist across the bacteria including inErythrobacterspecies derived from different environments. This strongly indicates that this novel thiosulfate oxidation pathway might be frequently used by microbes and plays an important role in the biogeochemical sulfur cycle in nature. |
| DOI | 10.1038/s41396-020-0684-5 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; China Ocean Mineral Resources R&D Association Grant[DY135-B2-14] ; National Key R and D Program of China[2018YFC0310800] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; Qingdao Innovation Leadership Program[18-1-2-7-zhc] ; CAS Interdisciplinary Innovation Team[JCTD-2018-12] ; Open Research Project of National Major Science & Technology Infrastructure (RV KEXUE)[NMSTI-KEXUE2017K01] |
| WOS研究方向 | Environmental Sciences & Ecology ; Microbiology |
| WOS类目 | Ecology ; Microbiology |
| WOS记录号 | WOS:000539443900002 |
| 出版者 | NATURE PUBLISHING GROUP |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167712 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Chaomin |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Geol & Environm, Qingdao, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋研究所; 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Zhang, Jing,Liu, Rui,Xi, Shichuan,et al. A novel bacterial thiosulfate oxidation pathway provides a new clue about the formation of zero-valent sulfur in deep sea[J]. ISME JOURNAL,2020:14. |
| APA | Zhang, Jing,Liu, Rui,Xi, Shichuan,Cai, Ruining,Zhang, Xin,&Sun, Chaomin.(2020).A novel bacterial thiosulfate oxidation pathway provides a new clue about the formation of zero-valent sulfur in deep sea.ISME JOURNAL,14. |
| MLA | Zhang, Jing,et al."A novel bacterial thiosulfate oxidation pathway provides a new clue about the formation of zero-valent sulfur in deep sea".ISME JOURNAL (2020):14. |
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| A novel bacterial th（3141KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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