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In vitro and in vivo anti-inflammatory activities of a fucose-rich fucoidan isolated from Saccharina japonica
Ni, Liying1; Wang, Lei2,3; Fu, Xiaoting1; Duan, Delin4,5; Jeon, You-Jin2,3; Xu, Jiachao1; Gao, Xin1
2020-08-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume156Pages:717-729
Corresponding AuthorFu, Xiaoting(xiaotingfu@ouc.edu.cn)
AbstractA fucoidan (LJSF4) purified from Saccharina japonica was found to show a strong anti-inflammatory effect via activity assay in lipopolysaccharide (LPS) induced RAW 264.7 macrophage cells and zebrafish. Chemical and structural analysis indicated that LJSF4 with a sulfate content of 30.72% was composed of fucose, galactose, rhamnose, xylose and mannose with molar ratio percentages of 79.49%, 16.76%, 0.82%, 1.08% and 1.84%. NMR spectroscopy showed that LJSF4 is a polysaccharide with a backbone of alternating 1 -> 3 linked alpha-L-fucopyranosyl and -> 4-alpha-L-fucopyranosyl with sulfate groups mainly at C-4 and partially at C-2 positions. Moreover, it also contained branches in the form of beta-D-Galp-(1 -> 4) units. The results of anti-inflammatory effect in vitro demonstrated that LJSF4 decreased the production of nitric oxide (NO) and cytokines, including TNF-alpha, IL-1 beta and IL-6. The mechanism revealed to be associated with the down-regulated expression of signal pathways including MAPK and NF-kappa B. By in vivo assay, LJSF4 showed a significantly protective effect by reducing the cell death rate, and the production of NO and ROS on LPS exposed zebrafish. Our results indicated that LJSF4 has the potential to be developed as an anti-inflammatory agent applied in functional food and cosmetic industries. (C) 2020 Elsevier B.V. All rights reserved.
KeywordSaccharine japonica Fucoidan Structure Anti-inflammation RAW 264.7 Zebrafish
DOI10.1016/j.ijbiomac.2020.04.012
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFD0901104] ; Major Scientific and Technological Innovation Project of Shandong Province[2019JZZY020613]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000538104200075
PublisherELSEVIER
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167652
Collection实验海洋生物学重点实验室
Corresponding AuthorFu, Xiaoting
Affiliation1.Ocean Univ China, Coll Food Sci & Engn, 5th Yushan Rd, Qingdao 266003, Shandong, Peoples R China
2.Jeju Natl Univ, Dept Marine Life Sci, Jeju City 63243, Jeju Self Gover, South Korea
3.Jeju Natl Univ, Marine Sci Inst, Jeju City 63333, Jeju Self Gover, South Korea
4.State Key Lab Seaweed Bioact Subst, 1th Daxueyuan Rd, Qingdao 266400, Shandong, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7th Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Ni, Liying,Wang, Lei,Fu, Xiaoting,et al. In vitro and in vivo anti-inflammatory activities of a fucose-rich fucoidan isolated from Saccharina japonica[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,156:717-729.
APA Ni, Liying.,Wang, Lei.,Fu, Xiaoting.,Duan, Delin.,Jeon, You-Jin.,...&Gao, Xin.(2020).In vitro and in vivo anti-inflammatory activities of a fucose-rich fucoidan isolated from Saccharina japonica.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,156,717-729.
MLA Ni, Liying,et al."In vitro and in vivo anti-inflammatory activities of a fucose-rich fucoidan isolated from Saccharina japonica".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 156(2020):717-729.
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