IOCAS-IR  > 海洋环境腐蚀与与生物污损重点实验室
Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides
Qi, Peng1,2,3; Wang, Yingwen1,2,3,4; Zhang, Dun1,2,3; Sun, Yan1,2,3; Zheng, Laibao1,2,3,4
2020-07-01
Source PublicationANALYTICAL BIOCHEMISTRY
ISSN0003-2697
Volume600Pages:7
Corresponding AuthorQi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
AbstractIn this paper, we present a facile and rapid multichannel approach for the simultaneous detection and discrimination of multiple bacterial species. The proposed assay employed four short antimicrobial peptides (SAMPs) for recognition due to their disparity in antibacterial activity against different bacterial strains, and utilized fluorescence measurements to explicate the bacterial recognition and disintegration disparity of the SAMPs. Then, linear discriminant analysis (LDA) was used to effectively discriminate and classify the observed characteristic fluorescence patterns of SAMPs towards target bacteria, exhibiting excellent bacterial discrimination and classification accuracy. This is the first report on the use of SAMPs as recognition units for simultaneous multiple bacterial detection and discrimination. The presented approach was simple, fast, highly repeatable, and required no labelling processes. According to the corresponding LDA discriminant results, six different target bacterial species could be effectively identified and discriminated within 30 min.
KeywordBacterial discrimination Short antimicrobial peptides Recognition Disintegration Linear discriminant analysis
DOI10.1016/j.ab.2020.113764
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41876101] ; Young Elite Scientists Sponsorship Program by CAST[20180276]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
WOS IDWOS:000537568700012
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167640
Collection海洋环境腐蚀与与生物污损重点实验室
Corresponding AuthorQi, Peng; Zhang, Dun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Qi, Peng,Wang, Yingwen,Zhang, Dun,et al. Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides[J]. ANALYTICAL BIOCHEMISTRY,2020,600:7.
APA Qi, Peng,Wang, Yingwen,Zhang, Dun,Sun, Yan,&Zheng, Laibao.(2020).Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides.ANALYTICAL BIOCHEMISTRY,600,7.
MLA Qi, Peng,et al."Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides".ANALYTICAL BIOCHEMISTRY 600(2020):7.
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