Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
| Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides | |
Qi, Peng1,2,3 ; Wang, Yingwen1,2,3,4; Zhang, Dun1,2,3; Sun, Yan1,2,3; Zheng, Laibao1,2,3,4
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| 2020-07-01 | |
| Source Publication | ANALYTICAL BIOCHEMISTRY
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| ISSN | 0003-2697 |
| Volume | 600Pages:7 |
| Corresponding Author | Qi, Peng(qipeng@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn) |
| Abstract | In this paper, we present a facile and rapid multichannel approach for the simultaneous detection and discrimination of multiple bacterial species. The proposed assay employed four short antimicrobial peptides (SAMPs) for recognition due to their disparity in antibacterial activity against different bacterial strains, and utilized fluorescence measurements to explicate the bacterial recognition and disintegration disparity of the SAMPs. Then, linear discriminant analysis (LDA) was used to effectively discriminate and classify the observed characteristic fluorescence patterns of SAMPs towards target bacteria, exhibiting excellent bacterial discrimination and classification accuracy. This is the first report on the use of SAMPs as recognition units for simultaneous multiple bacterial detection and discrimination. The presented approach was simple, fast, highly repeatable, and required no labelling processes. According to the corresponding LDA discriminant results, six different target bacterial species could be effectively identified and discriminated within 30 min. |
| Keyword | Bacterial discrimination Short antimicrobial peptides Recognition Disintegration Linear discriminant analysis |
| DOI | 10.1016/j.ab.2020.113764 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[41876101] ; Young Elite Scientists Sponsorship Program by CAST[20180276] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical |
| WOS ID | WOS:000537568700012 |
| Publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167640 |
| Collection | 海洋环境腐蚀与与生物污损重点实验室 |
| Corresponding Author | Qi, Peng; Zhang, Dun |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Qi, Peng,Wang, Yingwen,Zhang, Dun,et al. Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides[J]. ANALYTICAL BIOCHEMISTRY,2020,600:7. |
| APA | Qi, Peng,Wang, Yingwen,Zhang, Dun,Sun, Yan,&Zheng, Laibao.(2020).Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides.ANALYTICAL BIOCHEMISTRY,600,7. |
| MLA | Qi, Peng,et al."Multichannel bacterial discrimination based on recognition and disintegration disparity of short antimicrobial peptides".ANALYTICAL BIOCHEMISTRY 600(2020):7. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Multi-emitting fluor（2953KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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