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Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex
Ma, Yuzhen1,2,3; Yu, Huahua1,2; Liu, Weixiang1,2; Qin, Yukun1,2; Xing, Ronge1,2; Li, Pengcheng1,2
2020-07-15
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
卷号155页码:1491-1509
通讯作者Yu, Huahua(yuhuahua@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
摘要With wide application in agriculture, copper fungicides have undergone three stages of development: inorganic copper, synthetic organic copper, and natural organic copper. Using chitin/chitosan (CS) as a substrate, the natural organic copper fungicide C-coordinated O-carboxymethyl chitosan Cu(II) complex (O-CSLn-Cu) was developed in the laboratory. Taking Phytophthora capsici Leonian as an example, we explored the antifungal mechanism of O-CSLn-Cu by combining tandem mass tag (TMT)-based proteomics with non-targeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. A total of 1172 differentially expressed proteins were identified by proteomics analysis. According to the metabolomics analysis, 93 differentially metabolites were identified. Acetyl-CoA-related and membrane localized proteins showed significant differences in the proteomics analysis. Most of the differential expressed metabolites were distributed in the cytoplasm, followed by mitochondria. The integrated analysis revealed that O-CSLn-Cu could induce the "Warburg effect", with increased glycolysis in the cytoplasm and decreased metabolism in the mitochondria. Therefore, P. capsici Leonian had to compensate for ATP loss in the TCA cycle by increasing the glycolysis rate. However, this metabolic shift could not prevent the death of P. capsici Leonian. To verify this hypothesis, a series of biological experiments, such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), and enzyme activity measurements were carried out. The results suggest that O-CSLn-Cu causes mitochondrial injury, which consequently leads to excessive ROS levels and insufficient ATP levels, thereby killing P. capsici Leonian. (C) 2019 Elsevier B.V. All rights reserved.
关键词Natural organic-based copper O-CSLn-Cu Phytophthora capsici Leonian Proteomics-metabonomics integrated analysis Antifungal mechanism
DOI10.1016/j.ijbiomac.2019.11.127
收录类别SCI
语种英语
资助项目theNational Key Research and Development Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2018GHY115017] ; Key Research and Development Program of Shandong Province[2018GHY115008] ; Key Research and Development Program of Shandong Province[2019GHY112015]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号WOS:000536122500155
出版者ELSEVIER
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/167606
专题实验海洋生物学重点实验室
通讯作者Yu, Huahua; Li, Pengcheng
作者单位1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋大科学研究中心
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Ma, Yuzhen,Yu, Huahua,Liu, Weixiang,et al. Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,155:1491-1509.
APA Ma, Yuzhen,Yu, Huahua,Liu, Weixiang,Qin, Yukun,Xing, Ronge,&Li, Pengcheng.(2020).Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,155,1491-1509.
MLA Ma, Yuzhen,et al."Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 155(2020):1491-1509.
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