Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex | |
Ma, Yuzhen1,2,3; Yu, Huahua1,2 ; Liu, Weixiang1,2; Qin, Yukun1,2; Xing, Ronge1,2; Li, Pengcheng1,2
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| 2020-07-15 | |
| Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| Volume | 155Pages:1491-1509 |
| Corresponding Author | Yu, Huahua(yuhuahua@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn) |
| Abstract | With wide application in agriculture, copper fungicides have undergone three stages of development: inorganic copper, synthetic organic copper, and natural organic copper. Using chitin/chitosan (CS) as a substrate, the natural organic copper fungicide C-coordinated O-carboxymethyl chitosan Cu(II) complex (O-CSLn-Cu) was developed in the laboratory. Taking Phytophthora capsici Leonian as an example, we explored the antifungal mechanism of O-CSLn-Cu by combining tandem mass tag (TMT)-based proteomics with non-targeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. A total of 1172 differentially expressed proteins were identified by proteomics analysis. According to the metabolomics analysis, 93 differentially metabolites were identified. Acetyl-CoA-related and membrane localized proteins showed significant differences in the proteomics analysis. Most of the differential expressed metabolites were distributed in the cytoplasm, followed by mitochondria. The integrated analysis revealed that O-CSLn-Cu could induce the "Warburg effect", with increased glycolysis in the cytoplasm and decreased metabolism in the mitochondria. Therefore, P. capsici Leonian had to compensate for ATP loss in the TCA cycle by increasing the glycolysis rate. However, this metabolic shift could not prevent the death of P. capsici Leonian. To verify this hypothesis, a series of biological experiments, such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), and enzyme activity measurements were carried out. The results suggest that O-CSLn-Cu causes mitochondrial injury, which consequently leads to excessive ROS levels and insufficient ATP levels, thereby killing P. capsici Leonian. (C) 2019 Elsevier B.V. All rights reserved. |
| Keyword | Natural organic-based copper O-CSLn-Cu Phytophthora capsici Leonian Proteomics-metabonomics integrated analysis Antifungal mechanism |
| DOI | 10.1016/j.ijbiomac.2019.11.127 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | theNational Key Research and Development Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2018GHY115017] ; Key Research and Development Program of Shandong Province[2018GHY115008] ; Key Research and Development Program of Shandong Province[2019GHY112015] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| WOS ID | WOS:000536122500155 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167606 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Yu, Huahua; Li, Pengcheng |
| Affiliation | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Corresponding Author Affilication | Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Ma, Yuzhen,Yu, Huahua,Liu, Weixiang,et al. Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,155:1491-1509. |
| APA | Ma, Yuzhen,Yu, Huahua,Liu, Weixiang,Qin, Yukun,Xing, Ronge,&Li, Pengcheng.(2020).Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,155,1491-1509. |
| MLA | Ma, Yuzhen,et al."Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 155(2020):1491-1509. |
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| Integrated proteomic（13837KB） | 期刊论文 | 出版稿 | 延迟开放 | CC BY-NC-SA | View 2023-7-1后可获取 | |
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