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Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex
Ma, Yuzhen1,2,3; Yu, Huahua1,2; Liu, Weixiang1,2; Qin, Yukun1,2; Xing, Ronge1,2; Li, Pengcheng1,2
2020-07-15
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume155Pages:1491-1509
Corresponding AuthorYu, Huahua(yuhuahua@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
AbstractWith wide application in agriculture, copper fungicides have undergone three stages of development: inorganic copper, synthetic organic copper, and natural organic copper. Using chitin/chitosan (CS) as a substrate, the natural organic copper fungicide C-coordinated O-carboxymethyl chitosan Cu(II) complex (O-CSLn-Cu) was developed in the laboratory. Taking Phytophthora capsici Leonian as an example, we explored the antifungal mechanism of O-CSLn-Cu by combining tandem mass tag (TMT)-based proteomics with non-targeted liquid chromatography-mass spectrometry (LC-MS)-based metabolomics. A total of 1172 differentially expressed proteins were identified by proteomics analysis. According to the metabolomics analysis, 93 differentially metabolites were identified. Acetyl-CoA-related and membrane localized proteins showed significant differences in the proteomics analysis. Most of the differential expressed metabolites were distributed in the cytoplasm, followed by mitochondria. The integrated analysis revealed that O-CSLn-Cu could induce the "Warburg effect", with increased glycolysis in the cytoplasm and decreased metabolism in the mitochondria. Therefore, P. capsici Leonian had to compensate for ATP loss in the TCA cycle by increasing the glycolysis rate. However, this metabolic shift could not prevent the death of P. capsici Leonian. To verify this hypothesis, a series of biological experiments, such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), and enzyme activity measurements were carried out. The results suggest that O-CSLn-Cu causes mitochondrial injury, which consequently leads to excessive ROS levels and insufficient ATP levels, thereby killing P. capsici Leonian. (C) 2019 Elsevier B.V. All rights reserved.
KeywordNatural organic-based copper O-CSLn-Cu Phytophthora capsici Leonian Proteomics-metabonomics integrated analysis Antifungal mechanism
DOI10.1016/j.ijbiomac.2019.11.127
Indexed BySCI
Language英语
Funding ProjecttheNational Key Research and Development Program of China[2018YFC0311305] ; Key Research and Development Program of Shandong Province[2018GHY115017] ; Key Research and Development Program of Shandong Province[2018GHY115008] ; Key Research and Development Program of Shandong Province[2019GHY112015]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000536122500155
PublisherELSEVIER
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167606
Collection实验海洋生物学重点实验室
Corresponding AuthorYu, Huahua; Li, Pengcheng
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
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Ma, Yuzhen,Yu, Huahua,Liu, Weixiang,et al. Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2020,155:1491-1509.
APA Ma, Yuzhen,Yu, Huahua,Liu, Weixiang,Qin, Yukun,Xing, Ronge,&Li, Pengcheng.(2020).Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,155,1491-1509.
MLA Ma, Yuzhen,et al."Integrated proteomics and metabolomics analysis reveals the antifungal mechanism of the C-coordinated O-carboxymethyl chitosan Cu (II) complex".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 155(2020):1491-1509.
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