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Effects of calcein incorporation on benthic foraminiferal community under various concentrations and incubation durations
Li, Haotian1,5; Lei, Yanli1,4,5,6; Li, Tiegang2; Jian, Zhimin3
2020-05-01
Source PublicationMARINE MICROPALEONTOLOGY
ISSN0377-8398
Volume157Pages:12
Corresponding AuthorLei, Yanli(leiyanli@qdio.ac.cn) ; Jian, Zhimin(jian@tongji.edu.cn)
AbstractCalcein is widely used to mark the new calcium carbonate structures in foraminifera, but it remains unclear whether calcein has effects on the biological features (i.e., abundance, size). Here the biological effects of calcein on benthic foraminifera were investigated using a two-factor crossed experiment in which foraminiferal communities (10,393 live specimens in total) were cultured in seawater spiked with calcein at four concentrations (control, 1, 5, and 10 mg/L) and for three incubation durations (2, 4, and 6 weeks). Each treatment was performed in triplicate, yielding 36 samples (4 concentrations x 3 durations x 3 replicates). Results showed that high calcein concentrations (10 mg/L) and long incubation times (6 weeks) significantly affected the abundance, and the Margalef and Shannon-Wiener diversity indices, of foraminiferal communities. Dominant species showed a species-specific response to calcein. Interestingly, different foraminiferal test types showed different responses to calcein: the proportion of miliolids significantly increased in all calcein treatments (1, 5, and 10 mg/L) relative to the control (without calcein), in contrast to the proportion of rotaliids. Thus, calcein might have a positive effect on foraminiferal communities by enhancing porcelaneous groups. Given that high calcein concentrations and long incubation times can have negative effects on foraminifera, we suggest a relatively safe manipulation: at the beginning of a culture experiment, calcein (approximate to 5 mg/L) is added as an initial marker, then replaced with natural seawater halfway through the experiment. About 4 weeks before the end of the incubation, calcein can again be added to cultures.
KeywordCalcein concentration Culture experiment Foraminiferal biology Safe manipulation Micropaleontology Paleoceanography
DOI10.1016/j.marmicro.2020.101874
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41976058] ; National Natural Science Foundation of China[U1906211] ; Monitoring and Protection of Ecology and Environment in the East Pacific Ocean[DY135-E2-5] ; Taishan Scholars Project ; Senior User Project of RV KEXUE[KUEXUE2018G27]
WOS Research AreaPaleontology
WOS SubjectPaleontology
WOS IDWOS:000535536000008
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167600
Collection海洋生物分类与系统演化实验室
Corresponding AuthorLei, Yanli; Jian, Zhimin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China
2.MNR, Key Lab Marine Sedimentol & Environm Geol, Inst Oceanog 1, Qingdao 266061, Peoples R China
3.Tongji Univ, State Key Lab Marine Geol, Shanghai 200092, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Haotian,Lei, Yanli,Li, Tiegang,et al. Effects of calcein incorporation on benthic foraminiferal community under various concentrations and incubation durations[J]. MARINE MICROPALEONTOLOGY,2020,157:12.
APA Li, Haotian,Lei, Yanli,Li, Tiegang,&Jian, Zhimin.(2020).Effects of calcein incorporation on benthic foraminiferal community under various concentrations and incubation durations.MARINE MICROPALEONTOLOGY,157,12.
MLA Li, Haotian,et al."Effects of calcein incorporation on benthic foraminiferal community under various concentrations and incubation durations".MARINE MICROPALEONTOLOGY 157(2020):12.
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