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Transcriptome Analysis of Paralichthys olivaceus Erythrocytes Reveals Profound Immune Responses Induced by Edwardsiella tarda Infection
Sun, Bin1,2,3; Li, Xuepeng1; Ning, Xianhui1; Sun, Li1,2
2020-05-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume21Issue:9Pages:18
Corresponding AuthorSun, Li(lsun@qdio.ac.cn)
AbstractUnlike mammalian red blood cells (RBCs), fish RBCs are nucleated and thus capable of gene expression. Japanese flounder (Paralichthys olivaceus) is a species of marine fish with important economic values. Flounder are susceptible to Edwardsiella tarda, a severe bacterial pathogen that is able to infect and survive in flounder phagocytes. However, the infectivity of and the immune response induced by E. tarda in flounder RBCs are unclear. In the present research, we found that E. tarda was able to invade and replicate inside flounder RBCs in both in vitro and in vivo infections. To investigate the immune response induced by E. tarda in RBCs, transcriptome analysis of the spleen RBCs of flounder challenged with E. tarda was performed. Six sequencing libraries were constructed, and an average of 43 million clean reads per library were obtained, with 85% of the reads being successfully mapped to the genome of flounder. A total of 1720 differentially expressed genes (DEGs) were identified in E. tarda-infected fish. The DEGs were significantly enriched in diverse Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, especially those associated with immunity, disease, and infection. Ninety-one key DEGs involved in 12 immune-related pathways were found to form extensive interaction networks. Twenty-one genes that constituted the hub of the networks were further identified, which were highly regulated by E. tarda and involved in a number of immune processes, notably pathogen recognition and signal transduction, antigen processing and presentation, inflammation, and splicing. These results provide new insights into the immune role of flounder RBCs during bacterial infection.
KeywordParalichthys olivaceus red blood cells Edwardsiella tarda transcriptome immune response
DOI10.3390/ijms21093094
Indexed BySCI
Language英语
Funding ProjectMarine S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Key R&D Project of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000535581700065
PublisherMDPI
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167591
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1Wenhai Rd, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Bin,Li, Xuepeng,Ning, Xianhui,et al. Transcriptome Analysis of Paralichthys olivaceus Erythrocytes Reveals Profound Immune Responses Induced by Edwardsiella tarda Infection[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(9):18.
APA Sun, Bin,Li, Xuepeng,Ning, Xianhui,&Sun, Li.(2020).Transcriptome Analysis of Paralichthys olivaceus Erythrocytes Reveals Profound Immune Responses Induced by Edwardsiella tarda Infection.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(9),18.
MLA Sun, Bin,et al."Transcriptome Analysis of Paralichthys olivaceus Erythrocytes Reveals Profound Immune Responses Induced by Edwardsiella tarda Infection".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.9(2020):18.
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