Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Transcriptome Analysis of Paralichthys olivaceus Erythrocytes Reveals Profound Immune Responses Induced by Edwardsiella tarda Infection | |
Sun, Bin1,2,3 ; Li, Xuepeng1; Ning, Xianhui1; Sun, Li1,2
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| 2020-05-01 | |
| Source Publication | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| Volume | 21Issue:9Pages:18 |
| Corresponding Author | Sun, Li(lsun@qdio.ac.cn) |
| Abstract | Unlike mammalian red blood cells (RBCs), fish RBCs are nucleated and thus capable of gene expression. Japanese flounder (Paralichthys olivaceus) is a species of marine fish with important economic values. Flounder are susceptible to Edwardsiella tarda, a severe bacterial pathogen that is able to infect and survive in flounder phagocytes. However, the infectivity of and the immune response induced by E. tarda in flounder RBCs are unclear. In the present research, we found that E. tarda was able to invade and replicate inside flounder RBCs in both in vitro and in vivo infections. To investigate the immune response induced by E. tarda in RBCs, transcriptome analysis of the spleen RBCs of flounder challenged with E. tarda was performed. Six sequencing libraries were constructed, and an average of 43 million clean reads per library were obtained, with 85% of the reads being successfully mapped to the genome of flounder. A total of 1720 differentially expressed genes (DEGs) were identified in E. tarda-infected fish. The DEGs were significantly enriched in diverse Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, especially those associated with immunity, disease, and infection. Ninety-one key DEGs involved in 12 immune-related pathways were found to form extensive interaction networks. Twenty-one genes that constituted the hub of the networks were further identified, which were highly regulated by E. tarda and involved in a number of immune processes, notably pathogen recognition and signal transduction, antigen processing and presentation, inflammation, and splicing. These results provide new insights into the immune role of flounder RBCs during bacterial infection. |
| Keyword | Paralichthys olivaceus red blood cells Edwardsiella tarda transcriptome immune response |
| DOI | 10.3390/ijms21093094 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Marine S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-2] ; National Key R&D Project of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| WOS ID | WOS:000535581700065 |
| Publisher | MDPI |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167591 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Sun, Li |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Sun, Bin,Li, Xuepeng,Ning, Xianhui,et al. Transcriptome Analysis of Paralichthys olivaceus Erythrocytes Reveals Profound Immune Responses Induced by Edwardsiella tarda Infection[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(9):18. |
| APA | Sun, Bin,Li, Xuepeng,Ning, Xianhui,&Sun, Li.(2020).Transcriptome Analysis of Paralichthys olivaceus Erythrocytes Reveals Profound Immune Responses Induced by Edwardsiella tarda Infection.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(9),18. |
| MLA | Sun, Bin,et al."Transcriptome Analysis of Paralichthys olivaceus Erythrocytes Reveals Profound Immune Responses Induced by Edwardsiella tarda Infection".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.9(2020):18. |
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| File Name/Size | DocType | Version | Access | License | ||
| Transcriptome Analys（2640KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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