Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Effects of Lactobacillus pentosus HC-2 on the growth performance, intestinal morphology, immune-related genes and intestinal microbiota of Penaeus vannamei affected by aflatoxin B1 | |
Fang, Han1,3 ; Wang, Baojie1,2,4; Jiang, Keyong1,2,4; Liu, Mei1,2,4; Wang, Lei1,2,4
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| 2020-08-30 | |
| Source Publication | AQUACULTURE
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| ISSN | 0044-8486 |
| Volume | 525Pages:8 |
| Abstract | Lactobacillus pentosus HC-2 is a lactobacillus previous isolated and identified from aquatic animals (such as the goby, Acanthogobius hasta), which has shown a positive effect on growth and disease resistance in the Pacific white shrimp, Penaeus vannamei. Aflatoxin B1 (AFB1) is a highly carcinogenic and teratogenic toxin found in moldy feeds. This study aims to investigate the positive effect of L. pentosus on protecting the intestine of P. vannamei affected by AFB1 toxicity. Shrimp were respectively fed with formulated feed containing AFB1 (500 mu g/kg), AFB1 (500 mu g/kg) + L. pentosus HC-2 (5 x 10(8) CFU/g feed), and a basal commercial diet (Control group). After feeding for 6 weeks, the survival rate (AFB1 group: 37%, AFB1 + L. pentosus HC-2 group: 49%,Control group:61%) and the percent weight gain (AFB1 group: 98.28%, AFB1 + L. pentosus HC-2 group: 112.40%,Control group:111.27%) in the AFB1 + L. pentosus HC-2 group were higher than the AFB1 group. The intestinal morphology of the AFB1 + L. pentosus HC-2 group was more complete than that of the AFB1 group. The relative expression of immune-related genes including Rab, GST, mucin-like-PM, Dorsal, Relish and Pro-PO was detected. Expression in the experimental group significantly decreased compared with the control group, while the expression levels of immune genes (Rab, GST, mucin-like-PM, Dorsal and Pro-PO) in AFB1 + L. pentosus HC-2 group was higher than the AFB1 group. Intestinal microbiota analysis demonstrated that the community structure of intestinal microbiota was markedly different between the control group and experimental group. Comparing different genera of bacteria, the abundance of Proteobacteria in the AFB1 + L. pentosus HC-2 group increased significantly compared with the control group, while the abundance of Firmicutes and Bacteroidetes decreased. These results indicated that L. pentosus HC-2 had a positive effect on the growth performance and resistance of P. vannamei affected by AFB1 according to regulate the structure of intestinal flora. In breeding practice, probiotics can be used to reduce the harm of aflatoxin. This may be related to the probiotic's contribution to maintaining the structural integrity of the intestinal mucosa, promoting the expression of immune related genes and regulating the composition of intestinal microflora. |
| Keyword | Penaeus vannamei Lactobacillus pentosus HC-2 Aflatoxin B1 Growth performance Intestinal mucosa and intestinal microbiota |
| DOI | 10.1016/j.aquaculture.2020.735289 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key R&D Program of China[2019YFD0900401] ; Major Scientific and Technological Innovation Project, Shandong Province, China[2018SDKJ0502-2] |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000535434900005 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Version | 出版稿 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167588 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Wang, Lei |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences; Center for Ocean Mega-Science, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Fang, Han,Wang, Baojie,Jiang, Keyong,et al. Effects of Lactobacillus pentosus HC-2 on the growth performance, intestinal morphology, immune-related genes and intestinal microbiota of Penaeus vannamei affected by aflatoxin B1[J]. AQUACULTURE,2020,525:8. |
| APA | Fang, Han,Wang, Baojie,Jiang, Keyong,Liu, Mei,&Wang, Lei.(2020).Effects of Lactobacillus pentosus HC-2 on the growth performance, intestinal morphology, immune-related genes and intestinal microbiota of Penaeus vannamei affected by aflatoxin B1.AQUACULTURE,525,8. |
| MLA | Fang, Han,et al."Effects of Lactobacillus pentosus HC-2 on the growth performance, intestinal morphology, immune-related genes and intestinal microbiota of Penaeus vannamei affected by aflatoxin B1".AQUACULTURE 525(2020):8. |
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| Effects of Lactobaci（1201KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | View | |
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