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Effects of Lactobacillus pentosus HC-2 on the growth performance, intestinal morphology, immune-related genes and intestinal microbiota of Penaeus vannamei affected by aflatoxin B1
Fang, Han1,3; Wang, Baojie1,2,4; Jiang, Keyong1,2,4; Liu, Mei1,2,4; Wang, Lei1,2,4
2020-08-30
Source PublicationAQUACULTURE
ISSN0044-8486
Volume525Pages:8
AbstractLactobacillus pentosus HC-2 is a lactobacillus previous isolated and identified from aquatic animals (such as the goby, Acanthogobius hasta), which has shown a positive effect on growth and disease resistance in the Pacific white shrimp, Penaeus vannamei. Aflatoxin B1 (AFB1) is a highly carcinogenic and teratogenic toxin found in moldy feeds. This study aims to investigate the positive effect of L. pentosus on protecting the intestine of P. vannamei affected by AFB1 toxicity. Shrimp were respectively fed with formulated feed containing AFB1 (500 mu g/kg), AFB1 (500 mu g/kg) + L. pentosus HC-2 (5 x 10(8) CFU/g feed), and a basal commercial diet (Control group). After feeding for 6 weeks, the survival rate (AFB1 group: 37%, AFB1 + L. pentosus HC-2 group: 49%,Control group:61%) and the percent weight gain (AFB1 group: 98.28%, AFB1 + L. pentosus HC-2 group: 112.40%,Control group:111.27%) in the AFB1 + L. pentosus HC-2 group were higher than the AFB1 group. The intestinal morphology of the AFB1 + L. pentosus HC-2 group was more complete than that of the AFB1 group. The relative expression of immune-related genes including Rab, GST, mucin-like-PM, Dorsal, Relish and Pro-PO was detected. Expression in the experimental group significantly decreased compared with the control group, while the expression levels of immune genes (Rab, GST, mucin-like-PM, Dorsal and Pro-PO) in AFB1 + L. pentosus HC-2 group was higher than the AFB1 group. Intestinal microbiota analysis demonstrated that the community structure of intestinal microbiota was markedly different between the control group and experimental group. Comparing different genera of bacteria, the abundance of Proteobacteria in the AFB1 + L. pentosus HC-2 group increased significantly compared with the control group, while the abundance of Firmicutes and Bacteroidetes decreased. These results indicated that L. pentosus HC-2 had a positive effect on the growth performance and resistance of P. vannamei affected by AFB1 according to regulate the structure of intestinal flora. In breeding practice, probiotics can be used to reduce the harm of aflatoxin. This may be related to the probiotic's contribution to maintaining the structural integrity of the intestinal mucosa, promoting the expression of immune related genes and regulating the composition of intestinal microflora.
KeywordPenaeus vannamei Lactobacillus pentosus HC-2 Aflatoxin B1 Growth performance Intestinal mucosa and intestinal microbiota
DOI10.1016/j.aquaculture.2020.735289
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2019YFD0900401] ; Major Scientific and Technological Innovation Project, Shandong Province, China[2018SDKJ0502-2]
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000535434900005
PublisherELSEVIER
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/167588
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Fang, Han,Wang, Baojie,Jiang, Keyong,et al. Effects of Lactobacillus pentosus HC-2 on the growth performance, intestinal morphology, immune-related genes and intestinal microbiota of Penaeus vannamei affected by aflatoxin B1[J]. AQUACULTURE,2020,525:8.
APA Fang, Han,Wang, Baojie,Jiang, Keyong,Liu, Mei,&Wang, Lei.(2020).Effects of Lactobacillus pentosus HC-2 on the growth performance, intestinal morphology, immune-related genes and intestinal microbiota of Penaeus vannamei affected by aflatoxin B1.AQUACULTURE,525,8.
MLA Fang, Han,et al."Effects of Lactobacillus pentosus HC-2 on the growth performance, intestinal morphology, immune-related genes and intestinal microbiota of Penaeus vannamei affected by aflatoxin B1".AQUACULTURE 525(2020):8.
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