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邱楚雯; 刘梅; 王宝杰; 蒋克勇; 孙姝娟; 孟晓林; 骆作勇; 王雷
Source Publication海洋科学
Abstract为研究中国明对虾(Fenneropenaeus chinensis)血蓝蛋白C末端(FcHC-C)的抗菌功能,将血蓝蛋白基因FcHC的2个C末端基因片段连接到毕赤酵母表达载体pPIC9K中,构建酵母表达载体pPIC9K/FcHC-C。该载体经Sal I酶切后,采用PEG法转化毕赤酵母(Pichia pastoris GS115)。转化子经过PCR鉴定后,阳性克隆通过含有G418的YPD平板筛选,获得高拷贝重组子。重组毕赤酵母利用甲醇诱导表达目的基因。经Tricine-SDS-PAGE和Western blot分析结果表明,利用酵母工程菌成功表达了血蓝蛋白C末端片段(rFcHC-C1和rFcHC...
Keyword中国明对虾(Fenneropenaeus Chinensis) 血蓝蛋白 抗菌肽 毕赤酵母(Pichia Pastoris) 重组表达
Document Type期刊论文
Recommended Citation
GB/T 7714
邱楚雯,刘梅,王宝杰,等. 中国明对虾血蓝蛋白C末端片段在毕赤酵母中的表达及其抗菌活性[J]. 海洋科学,2013(6):41646.
APA 邱楚雯.,刘梅.,王宝杰.,蒋克勇.,孙姝娟.,...&王雷.(2013).中国明对虾血蓝蛋白C末端片段在毕赤酵母中的表达及其抗菌活性.海洋科学(6),41646.
MLA 邱楚雯,et al."中国明对虾血蓝蛋白C末端片段在毕赤酵母中的表达及其抗菌活性".海洋科学 .6(2013):41646.
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