Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells

doi:10.1007/s00343-019-9129-2

IOCAS-IR > 实验海洋生物学重点实验室

	Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells
	Zhou Shengnan 1; Liu Ge 2,3; Wu Shimei 1
	2020-05-01
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
卷号	38 期号:3 页码:826-833
通讯作者	Wu Shimei(shimeiwu2016@126.com)
摘要	Marine bacterial strain Bacillus sp. CS30 exhibited high anticancer activity against Huh7.5 human liver cancer. We purified the corresponding anticancer agent by sequential acidic precipitation, methanol extraction, Sephadex LH-20 chromatography, and reversed phase high-performance liquid chromatography (RP-HPLC), then analyzed it in mass spectrometry. Based on the results of purification and mass spectrometry, we deduced that the anticancer agent was the same component as our previously purified antifungal agent surfactin CS30-2. However, to the best of our knowledge, this is the first report on the surfactin possessing both antifungal and anticancer activities. Surfactin CS30-2 was demonstrated to exhibit high anticancer activity in a dose-dependent manner against Huh7.5 liver cancer cells. Further investigation showed that surfactin CS30-2 induced the increased generation of reactive oxygen species (ROS) and severe disruption of cell membrane, thus leading to cell death. However, unlike previously reported surfactins, surfactin CS30-2 caused cancer cell death via necrosis instead of apoptosis.
关键词	Bacillus surfactin anticancer activity reactive oxygen species (ROS)
DOI	10.1007/s00343-019-9129-2
收录类别	SCI
语种	英语
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000536606000020
出版者	SCIENCE PRESS
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167558
专题	实验海洋生物学重点实验室
通讯作者	Wu Shimei
作者单位	1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhou Shengnan,Liu Ge,Wu Shimei. Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(3):826-833.
APA	Zhou Shengnan,Liu Ge,&Wu Shimei.(2020).Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(3),826-833.
MLA	Zhou Shengnan,et al."Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.3(2020):826-833.

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