Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells | |
| Zhou Shengnan1; Liu Ge2,3; Wu Shimei1 | |
| 2020-05-01 | |
| Source Publication | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| ISSN | 2096-5508 |
| Volume | 38Issue:3Pages:826-833 |
| Corresponding Author | Wu Shimei(shimeiwu2016@126.com) |
| Abstract | Marine bacterial strain Bacillus sp. CS30 exhibited high anticancer activity against Huh7.5 human liver cancer. We purified the corresponding anticancer agent by sequential acidic precipitation, methanol extraction, Sephadex LH-20 chromatography, and reversed phase high-performance liquid chromatography (RP-HPLC), then analyzed it in mass spectrometry. Based on the results of purification and mass spectrometry, we deduced that the anticancer agent was the same component as our previously purified antifungal agent surfactin CS30-2. However, to the best of our knowledge, this is the first report on the surfactin possessing both antifungal and anticancer activities. Surfactin CS30-2 was demonstrated to exhibit high anticancer activity in a dose-dependent manner against Huh7.5 liver cancer cells. Further investigation showed that surfactin CS30-2 induced the increased generation of reactive oxygen species (ROS) and severe disruption of cell membrane, thus leading to cell death. However, unlike previously reported surfactins, surfactin CS30-2 caused cancer cell death via necrosis instead of apoptosis. |
| Keyword | Bacillus surfactin anticancer activity reactive oxygen species (ROS) |
| DOI | 10.1007/s00343-019-9129-2 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Marine & Freshwater Biology ; Oceanography |
| WOS Subject | Limnology ; Oceanography |
| WOS ID | WOS:000536606000020 |
| Publisher | SCIENCE PRESS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/167558 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Wu Shimei |
| Affiliation | 1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China |
| Recommended Citation GB/T 7714 | Zhou Shengnan,Liu Ge,Wu Shimei. Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(3):826-833. |
| APA | Zhou Shengnan,Liu Ge,&Wu Shimei.(2020).Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(3),826-833. |
| MLA | Zhou Shengnan,et al."Marine bacterial surfactin CS30-2 induced necrosis-like cell death in Huh7.5 liver cancer cells".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.3(2020):826-833. |
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