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PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan
Yang Yue1,2,3; Xing Rong'e1,2; Liu Song1,2; Qin Yukun1,2; Li Kecheng1,2; Yu Huahua1,2; Li Pengcheng1,2
2020-05-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume38Issue:3Pages:834-840
Corresponding AuthorXing Rong'e(xingronge@qdio.ac.cn) ; Li Pengcheng()
AbstractWe previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan (HACC) promoted the production of nitric oxide (NO) and proinflammatory cytokines by activating the mitogen-activated protein kinases (MAPK) and Janus kinase (JAK)/STAT pathways in RAW 264.7 cells, indicating good immunomodulatory activity of HACC. In this study, to further investigate the immunomodulatory mechanisms of HACC, we determined the roles of phosphatidylinositol 3-kinase (PI3K)/Akt, activating protein (AP-1) and nuclear factor kappa B (NF-kappa B) in HACC-induced activation of RAW 264.7 cells by the western blotting. The results suggest that HACC promoted the phosphorylation of p85 and Akt. Furthermore, c-Jun and p65 were also increased after the treatment of RAW 264.7 cells with HACC, indicating the translocation of NF-kappa B and AP-1 from cytoplasm to nucleus. In addition, as scanning electron microscopy (SEM) analysis shows, the cell morphology changed after HACC treatment. These findings indicate that HACC activated MAPK, JAK/STAT, and PI3K/Akt signaling pathways dependent on AP-1 and NF-kappa B activation in RAW 264.7 cells, ultimately leading to the increase of NO and cytokines.
Keywordhydroxypropyltrimethyl ammonium chloride chitosan RAW 264 7 cells PI3K Akt pathway nuclear factor-kappa B activating protein 1
DOI10.1007/s00343-019-9013-0
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000536606000021
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167546
Collection实验海洋生物学重点实验室
Corresponding AuthorXing Rong'e; Li Pengcheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang Yue,Xing Rong'e,Liu Song,et al. PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(3):834-840.
APA Yang Yue.,Xing Rong'e.,Liu Song.,Qin Yukun.,Li Kecheng.,...&Li Pengcheng.(2020).PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(3),834-840.
MLA Yang Yue,et al."PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.3(2020):834-840.
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