Biosynthesis of acetylacetone inspired by its biodegradation

doi:10.1186/s13068-020-01725-9

IOCAS-IR

	Biosynthesis of acetylacetone inspired by its biodegradation
	Zhou, Yifei 1,2; Ding, Yamei 3; Gao, Wenjie 1; Wang, Jichao 1; Liu, Xiutao 1,2; Xian, Mo 1; Feng, Xinjun 1; Zhao, Guang 1,4
	2020-05-15
发表期刊	BIOTECHNOLOGY FOR BIOFUELS
卷号	13 期号:1 页码:10
通讯作者	Feng, Xinjun(fengxj@qibebt.ac.cn) ; Zhao, Guang(zhaoguang@qibebt.ac.cn)
摘要	Background Acetylacetone is a commercially bulk chemical with diverse applications. However, the traditional manufacturing methods suffer from many drawbacks such as multiple steps, harsh conditions, low yield, and environmental problems, which hamper further applications of petrochemical-based acetylacetone. Compared to conventional chemical methods, biosynthetic methods possess advantages such as being eco-friendly, and having mild conditions, high selectivity and low potential costs. It is urgent to develop biosynthetic route for acetylacetone to avoid the present problems. Results The biosynthetic pathway of acetylacetone was constructed by reversing its biodegradation route, and the acetylacetone was successfully produced by engineered Escherichia coli (E. coli) by overexpression of acetylacetone-cleaving enzyme (Dke1) from Acinetobacter johnsonii. Several promising amino acid residues were selected for enzyme improvement based on sequence alignment and structure analysis, and the acetylacetone production was improved by site-directed mutagenesis of Dke1. The double-mutant (K15Q/A60D) strain presented the highest acetylacetone-producing capacity which is 3.6-fold higher than that of the wild-type protein. Finally, the strain accumulated 556.3 +/- 15.2 mg/L acetylacetone in fed-batch fermentation under anaerobic conditions. Conclusions This study presents the first intuitive biosynthetic pathway for acetylacetone inspired by its biodegradation, and shows the potential for large-scale production.
关键词	Acetylacetone biosynthesis Acetylacetone-cleaving enzyme Rational design Site-directed mutagenesis beta-Dicarbonyl compounds
DOI	10.1186/s13068-020-01725-9
收录类别	SCI
语种	英语
资助项目	National Defense Science and Technology Innovation Zone Foundation of China ; National Natural Science Foundation of China[31722001] ; National Natural Science Foundation of China[31670089] ; National Natural Science Foundation of China[31800081] ; Taishan Scholars Program of Shandong Province[ts201712076] ; Natural Science Foundation of Shandong[JQ201707] ; Natural Science Foundation of Shandong[ZR2019QB015] ; Youth Innovation Promotion Association at the Chinese Academy of Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Energy & Fuels
WOS类目	Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:000536088100001
出版者	BMC
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167537
专题	中国科学院海洋研究所
通讯作者	Feng, Xinjun; Zhao, Guang
作者单位	1.Chinese Acad Sci, CAS Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Yifei,Ding, Yamei,Gao, Wenjie,et al. Biosynthesis of acetylacetone inspired by its biodegradation[J]. BIOTECHNOLOGY FOR BIOFUELS,2020,13(1):10.
APA	Zhou, Yifei.,Ding, Yamei.,Gao, Wenjie.,Wang, Jichao.,Liu, Xiutao.,...&Zhao, Guang.(2020).Biosynthesis of acetylacetone inspired by its biodegradation.BIOTECHNOLOGY FOR BIOFUELS,13(1),10.
MLA	Zhou, Yifei,et al."Biosynthesis of acetylacetone inspired by its biodegradation".BIOTECHNOLOGY FOR BIOFUELS 13.1(2020):10.

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