Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa

doi:10.1007/s00343-020-9304-5

	Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa
	Zhang, Qingchun 1,2,3; Niu, Zhuang 1,2,3,4; Wang, Jinxiu 1,2,3,4; Liu, Chao 1,2,3,4; Kong, Fanzhou1,2,3 ; Hu, Xiaokun 1,2,3,4; Zhao, Jiayu 1,2,3,4; Yu, Rencheng 1,2,3,4
	2020-05-09
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
页码	17
通讯作者	Yu, Rencheng(rcyu@qdio.ac.cn)
摘要	Phaeocystis globosa is an important harmful algal bloom causative species distributing widely in temperate and tropical coastal waters in the world. The morphological, physiological, and biochemical characteristics are different among geographic strains, which can not be distinguished with nuclear ribosomal DNA markers at present. Therefore, the genetic distance and phylogeographic relationships of nuclear 28S rDNA D1-D2 and ITS regions, and three chloroplast intergenic spacers (petN-trnS1, trnM1-psbA, and rbcS-rpl27) were analyzed and compared among 13 strains of P. globosa isolated from the Pacific Ocean and Atlantic Ocean in this study. In addition, the nucleotide polymorphisms of 28S rDNA D1-D2, ITS, and rbcS-rpl27 regions were evaluated in two P. globosa strains. The various levels of nucleotide polymorphism were in the nuclear 28S rDNA D1-D2 region and ITS region, but no polymorphism was in the chloroplast rbcS-rpl27 intergenic spacer. A reasonable intraspecific phylogeographic relationship was presented by rbcS-rpl27 intergenic spacer, which had the strongest distinction to geographic strains compared to those of 28S rDNA D1-D2 and ITS regions. In the phylogenetic tree of rbcS-rpl27 intergenic spacer, the two strains from the North Sea of the Atlantic Ocean were divided firstly from the species of P. globosa, and then formed an independent clade, while the other Atlantic strains and all of Pacific strains joined up to build the other clade. It was implied that at least two genetically distant populations of P. globosa existed in the Atlantic coastal regions. This study provided a high-resolution chloroplast marker to analyze intraspecific phylogeographic populations of P. globosa, and preliminarily clarified the genetic relationships of the Pacific and Atlantic strains of P. globosa.
关键词	Phaeocystis globosa chloroplast DNA marker phylogeny
DOI	10.1007/s00343-020-9304-5
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Plan Project ; Sino-Australian Centre for Healthy Coasts[2016YFE0101500] ; Science & Technology Basic Resources Investigation Program[2018FY100206] ; National Natural Science Foundation of China[41576121] ; National Natural Science Foundation of China[41776127]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000531220300004
出版者	SCIENCE PRESS
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167488
专题	海洋生态与环境科学重点实验室
通讯作者	Yu, Rencheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
通讯作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Zhang, Qingchun,Niu, Zhuang,Wang, Jinxiu,et al. Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020:17.
APA	Zhang, Qingchun.,Niu, Zhuang.,Wang, Jinxiu.,Liu, Chao.,Kong, Fanzhou.,...&Yu, Rencheng.(2020).Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,17.
MLA	Zhang, Qingchun,et al."Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2020):17.

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