IOCAS-IR  > 海洋生态与环境科学重点实验室
Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa
Zhang, Qingchun1,2,3; Niu, Zhuang1,2,3,4; Wang, Jinxiu1,2,3,4; Liu, Chao1,2,3,4; Kong, Fanzhou1,2,3; Hu, Xiaokun1,2,3,4; Zhao, Jiayu1,2,3,4; Yu, Rencheng1,2,3,4
2020-05-09
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Pages17
Corresponding AuthorYu, Rencheng(rcyu@qdio.ac.cn)
AbstractPhaeocystis globosa is an important harmful algal bloom causative species distributing widely in temperate and tropical coastal waters in the world. The morphological, physiological, and biochemical characteristics are different among geographic strains, which can not be distinguished with nuclear ribosomal DNA markers at present. Therefore, the genetic distance and phylogeographic relationships of nuclear 28S rDNA D1-D2 and ITS regions, and three chloroplast intergenic spacers (petN-trnS1, trnM1-psbA, and rbcS-rpl27) were analyzed and compared among 13 strains of P. globosa isolated from the Pacific Ocean and Atlantic Ocean in this study. In addition, the nucleotide polymorphisms of 28S rDNA D1-D2, ITS, and rbcS-rpl27 regions were evaluated in two P. globosa strains. The various levels of nucleotide polymorphism were in the nuclear 28S rDNA D1-D2 region and ITS region, but no polymorphism was in the chloroplast rbcS-rpl27 intergenic spacer. A reasonable intraspecific phylogeographic relationship was presented by rbcS-rpl27 intergenic spacer, which had the strongest distinction to geographic strains compared to those of 28S rDNA D1-D2 and ITS regions. In the phylogenetic tree of rbcS-rpl27 intergenic spacer, the two strains from the North Sea of the Atlantic Ocean were divided firstly from the species of P. globosa, and then formed an independent clade, while the other Atlantic strains and all of Pacific strains joined up to build the other clade. It was implied that at least two genetically distant populations of P. globosa existed in the Atlantic coastal regions. This study provided a high-resolution chloroplast marker to analyze intraspecific phylogeographic populations of P. globosa, and preliminarily clarified the genetic relationships of the Pacific and Atlantic strains of P. globosa.
KeywordPhaeocystis globosa chloroplast DNA marker phylogeny
DOI10.1007/s00343-020-9304-5
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Plan Project ; Sino-Australian Centre for Healthy Coasts[2016YFE0101500] ; Science & Technology Basic Resources Investigation Program[2018FY100206] ; National Natural Science Foundation of China[41576121] ; National Natural Science Foundation of China[41776127]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000531220300004
PublisherSCIENCE PRESS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167488
Collection海洋生态与环境科学重点实验室
Corresponding AuthorYu, Rencheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Recommended Citation
GB/T 7714
Zhang, Qingchun,Niu, Zhuang,Wang, Jinxiu,et al. Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020:17.
APA Zhang, Qingchun.,Niu, Zhuang.,Wang, Jinxiu.,Liu, Chao.,Kong, Fanzhou.,...&Yu, Rencheng.(2020).Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,17.
MLA Zhang, Qingchun,et al."Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2020):17.
Files in This Item:
File Name/Size DocType Version Access License
Development of high-(1827KB)期刊论文出版稿开放获取CC BY-NC-SAView
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Qingchun]'s Articles
[Niu, Zhuang]'s Articles
[Wang, Jinxiu]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Qingchun]'s Articles
[Niu, Zhuang]'s Articles
[Wang, Jinxiu]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Qingchun]'s Articles
[Niu, Zhuang]'s Articles
[Wang, Jinxiu]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Development of high-resolution chloroplast markers for intraspecific phylogeographic studies of Phaeocystis globosa.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.