A T3SS Regulator Mutant of Vibrio alginolyticus Affects Antibiotic Susceptibilities and Provides Significant Protection to Danio rerio as a Live Attenuated Vaccine

doi:10.3389/fcimb.2020.00183

IOCAS-IR > 实验海洋生物学重点实验室

	A T3SS Regulator Mutant of Vibrio alginolyticus Affects Antibiotic Susceptibilities and Provides Significant Protection to Danio rerio as a Live Attenuated Vaccine
	Zhou, Shihui 1,2,3,4,5,6; Tu, Xueting 1,2,3,4,5,6; Pang, Huanying 1,2,3,4,5,6; Hoare, Rowena 7; Monaghan, Sean J.7; Luo, Jiajun 2,3,4; Jian, Jichan 2,3,4,5,6
	2020-04-28
发表期刊	FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ISSN	2235-2988
卷号	10 页码:13
通讯作者	Pang, Huanying(phying1218@163.com)
摘要	Vibrio alginolyticus is a major cause of Vibriosis in farmed marine aquatic animals and has caused large economic losses to the Asian aquaculture industry in recent years. Therefore, it is necessary to control V. alginolyticus effectively. The virulence mechanism of V. alginolyticus, the Type III secretion system (T3SS), is closely related to its pathogenicity. In this study, the T3SS gene tyeA was cloned from V. alginolyticus wild-type strain HY9901 and the results showed that the deduced amino acid sequence of V. alginolyticus tyeA shared 75-83% homology with other Vibrio spp. The mutant strain HY9901 Delta tyeA was constructed by Overlap-PCR and homologous recombination techniques. The HY9901 Delta tyeA mutant exhibited an attenuated swarming phenotype and an similar to 40-fold reduction in virulence to zebrafish. However, the HY9901 Delta tyeA mutant showed no difference in growth, biofilm formation and ECPase activity. Antibiotic susceptibility test was observed that wild and mutant strains were extremely susceptible to Amikacin, Minocycline, Gentamicin, Cefperazone; and resistant to oxacillin, clindamycin, ceftazidime. In contrast wild strains are sensitive to tetracycline, chloramphenicol, kanamycin, doxycycline, while mutant strains are resistant to them. qRT-PCR was employed to analyze the transcription levels of T3SS-related genes, the results showed that compared with HY9901 wild type, Delta tyeA had increased expression of vscL, vscK, vscO, vopS, vopN, vscN, and hop. Following vaccination with the mutant strain, zebrafish had significantly higher survival than controls following infection with the wild-type HY9901 (71.2% relative percent survival; RPS). Analysis of immune gene expression by qPCR showed that vaccination with HY9901 Delta tyeA increased the expression of IgM, IL-1 beta, IL-6, and TNF-alpha in zebrafish. This study provides evidence of protective efficacy of a live attenuated vaccine targeting the T3SS of V. alginolyticus which may be facilitated by up-regulated pro-inflammatory and immunoglobulin-related genes.
关键词	Vibrio alginolyticus live attenuated vaccine type III secretory system tyeA regulator
DOI	10.3389/fcimb.2020.00183
收录类别	SCI
语种	英语
资助项目	Shenzhen Science and Technology Project[JCYJ20170818111629778] ; National Key Research and Development Program of China[2018YFD0900501] ; National Natural Science Foundation of China[31402344] ; National Natural Science Foundation of China[31670129] ; Natural Science Foundation of Guangdong Province[2017A030313174] ; Independent Project of SouthernMarine Science and Engineering Guangdong Laboratory (Zhanjiang)[ZJW-2019-05] ; special funds for science technology innovation and industrial development of Shenzhen Dapeng New District[PT202001-13]
WOS研究方向	Immunology ; Microbiology
WOS类目	Immunology ; Microbiology
WOS记录号	WOS:000533355300001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167479
专题	实验海洋生物学重点实验室
通讯作者	Pang, Huanying
作者单位	1.Guangdong Ocean Univ, Shenzhen Inst, Shenzhen, Peoples R China 2.Guangdong Ocean Univ, Fisheries Coll, Zhanjiang, Peoples R China 3.Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China 4.Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 7.Univ Stirling, Inst Aquaculture, Stirling, Scotland
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zhou, Shihui,Tu, Xueting,Pang, Huanying,et al. A T3SS Regulator Mutant of Vibrio alginolyticus Affects Antibiotic Susceptibilities and Provides Significant Protection to Danio rerio as a Live Attenuated Vaccine[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2020,10:13.
APA	Zhou, Shihui.,Tu, Xueting.,Pang, Huanying.,Hoare, Rowena.,Monaghan, Sean J..,...&Jian, Jichan.(2020).A T3SS Regulator Mutant of Vibrio alginolyticus Affects Antibiotic Susceptibilities and Provides Significant Protection to Danio rerio as a Live Attenuated Vaccine.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,10,13.
MLA	Zhou, Shihui,et al."A T3SS Regulator Mutant of Vibrio alginolyticus Affects Antibiotic Susceptibilities and Provides Significant Protection to Danio rerio as a Live Attenuated Vaccine".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 10(2020):13.

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