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Overexpression of T-bet, GATA-3 and TGF-ss Induces IFN-gamma, IL-4/13A, and IL-17A Expression in Atlantic Salmon
Slettjord, Tiril H.1,2; Sekkenes, Hege J.1,3; Chi, Heng1,4; Bogwald, Jarl1; Swain, Trilochan1; Dalmo, Roy A.1; Swain, Jaya Kumari1,5
2020-04-01
Source PublicationBIOLOGY-BASEL
Volume9Issue:4Pages:17
Corresponding AuthorDalmo, Roy A.(roy.dalmo@uit.no) ; Swain, Jaya Kumari(jaya.k.swain@uit.no)
AbstractThe overexpression of GATA-3, T-bet and TGF-ss may theoretically induce IL-4/A, IFN-gamma and IL-17A expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged T-bet, GATA-3 and TGF-ss were used as overexpression tools, transfected into cells or injected intramuscularly to monitor the expression of IFN-gamma, IL-4/13A and IL-17A. In addition, the fish were either experimentally challenged with Vibrio anguillarum (VA group) or Piscirickettsia salmonis (PS group). The reporter gene (RFP) inserted upstream of the GATA-3, T-bet and TGF-ss genes, was observed in muscle cell nuclei and in inflammatory cells after intramuscular (i.m.) injection. PS group: following the injection of GATA-3 and T-bet-encoding plasmids, the expression of GATA-3 and T-bet was high at the injection site. The spleen expression of IFN-gamma, following the injection of a T-bet-encoding plasmid, was significantly higher on day 2. VA group: The T-bet and GATA-3-overexpressing fish expressed high T-bet and GATA-3 mRNA levels in the muscles and on day 4 post-challenge. The expression of TGF-ss in the muscles of fish injected with TGF-ss-encoding plasmids was significantly higher on days 7 (8 days pre-challenge) and 19 (4 days after challenge). The protective effects of the overexpression of T-bet, GATA-3 and TGF-ss on both bacterial infections were negligible.
Keywordoverexpression T-bet GATA-3 TGF-ss Atlantic salmon gene expression
DOI10.3390/biology9040082
Indexed BySCI
Language英语
Funding ProjectResearch Council of Norway[239140] ; Research Council of Norway[237315] ; Research Council of Norway[183204] ; Tromso Research Foundation ; University of Tromso
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS IDWOS:000533889200002
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167437
Collection实验海洋生物学重点实验室
Corresponding AuthorDalmo, Roy A.; Swain, Jaya Kumari
Affiliation1.Univ Tromso, Art Univ Norway, Fac Biosci Fisheries & Econ, Norwegian Coll Fishery Sci, N-9037 Tromso, Norway
2.Cermaq Ltd, N-8286 Gjaerbakknes, Steigen, Norway
3.Val FoU AS, N-7970 Val, Kolvereid, Norway
4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
5.NOFIMA, Muninbakken 9-13, N-9291 Tromso, Norway
Recommended Citation
GB/T 7714
Slettjord, Tiril H.,Sekkenes, Hege J.,Chi, Heng,et al. Overexpression of T-bet, GATA-3 and TGF-ss Induces IFN-gamma, IL-4/13A, and IL-17A Expression in Atlantic Salmon[J]. BIOLOGY-BASEL,2020,9(4):17.
APA Slettjord, Tiril H..,Sekkenes, Hege J..,Chi, Heng.,Bogwald, Jarl.,Swain, Trilochan.,...&Swain, Jaya Kumari.(2020).Overexpression of T-bet, GATA-3 and TGF-ss Induces IFN-gamma, IL-4/13A, and IL-17A Expression in Atlantic Salmon.BIOLOGY-BASEL,9(4),17.
MLA Slettjord, Tiril H.,et al."Overexpression of T-bet, GATA-3 and TGF-ss Induces IFN-gamma, IL-4/13A, and IL-17A Expression in Atlantic Salmon".BIOLOGY-BASEL 9.4(2020):17.
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