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Molecular cloning, characterization and expression profiles of CD2AP in Nile tilapia (Oreochromis niloticus) responding to Streptococcus agalactiae infection and interaction with CD2 cytoplasmic segment
Li, Yuan1,2,3; Wang, Bei1,2,3,4,5; Wang, Zhi-wen1,2,3; Huang, Yu1,2,3,4,5; Jian, Ji-chang1,4; Lu, Yi-shan1,2,3,4,5
2020-06-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume101Pages:205-215
Corresponding AuthorWang, Bei(wong19820204@126.com) ; Lu, Yi-shan(fishdis@163.com)
AbstractThe interaction between CD2-associated protein (CD2AP) and CD2 plays a vital role in lymphocyte adhesion and T cells activation in mammals. In this study, a CD2AP gene (GenBank accession number: MK579862; designated as On-CD2AP) was identified from tilapia (Oreochromis niloticus). Sequence analysis showed that On-CD2AP protein shares high similarity with mammals, including three Src homology 3 (SH3) domains, a section of poly proline motif and a coiled coil region. Transcription levels of On-CD2AP were detected in nine tissues of healthy Nile tilapia, and the highest expression levels were detected in the spleen and gill. On-CD2AP were significantly up-regulated in thymus, head kidney and brain after infected by Streptococcus agalactiae, as well as in head kidney leukocytes (HKLs) with LPS and LTA stimulation. Moreover, a section conserved pro-rich motif that are responsible for binding of CD2 to CD2AP were found in the CD2 cytoplasmic sequence of Nile tilapia (On-CD2C). A weak interaction between On-CD2AP and On-CD2C was proved by yeast two-hybrid assay. In addition, the recombinant proteins of CD2AP-His (rOn-CD2AP-His) and GST-CD2C (GST-rOn-CD2C) were obtained through prokaryotic expression system. His pull-down assay showed that rOn-CD2AP-His and GST-rOn-CD2C could bind to each other. These findings indicate that CD2AP is crucial in immune response during S.agalactiae infection, and the mechanism of interaction between CD2AP and CD2 is conservative in Nile tilapia.
KeywordCD2-associated protein Nile tilapia CD2 Immune response T cells activation Streptococcus agalactiae
DOI10.1016/j.fsi.2020.03.054
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31702386] ; International Cooperation Science & Technology Planning Project of Guangdong Province of China[2017A050501037] ; Shenzhen Science and Technology Project[JCYJ20180306173022502]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000531054100022
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167422
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Bei; Lu, Yi-shan
Affiliation1.Guangdong Ocean Univ, Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen Inst, Shenzhen 518120, Guangdong, Peoples R China
2.Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Southern Marine Sci & Engn Guangdong Lab Zhanjian, Fisheries Coll, Zhanjiang, Peoples R China
3.Key Lab Control Dis Aquat Econ Anim Guangdong Hig, Zhanjiang, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Yuan,Wang, Bei,Wang, Zhi-wen,et al. Molecular cloning, characterization and expression profiles of CD2AP in Nile tilapia (Oreochromis niloticus) responding to Streptococcus agalactiae infection and interaction with CD2 cytoplasmic segment[J]. FISH & SHELLFISH IMMUNOLOGY,2020,101:205-215.
APA Li, Yuan,Wang, Bei,Wang, Zhi-wen,Huang, Yu,Jian, Ji-chang,&Lu, Yi-shan.(2020).Molecular cloning, characterization and expression profiles of CD2AP in Nile tilapia (Oreochromis niloticus) responding to Streptococcus agalactiae infection and interaction with CD2 cytoplasmic segment.FISH & SHELLFISH IMMUNOLOGY,101,205-215.
MLA Li, Yuan,et al."Molecular cloning, characterization and expression profiles of CD2AP in Nile tilapia (Oreochromis niloticus) responding to Streptococcus agalactiae infection and interaction with CD2 cytoplasmic segment".FISH & SHELLFISH IMMUNOLOGY 101(2020):205-215.
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