Molecular cloning, characterization and expression profiles of CD2AP in Nile tilapia (Oreochromis niloticus) responding to Streptococcus agalactiae infection and interaction with CD2 cytoplasmic segment

doi:10.1016/j.fsi.2020.03.054

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	Molecular cloning, characterization and expression profiles of CD2AP in Nile tilapia (Oreochromis niloticus) responding to Streptococcus agalactiae infection and interaction with CD2 cytoplasmic segment
	Li, Yuan 1,2,3; Wang, Bei1,2,3,4,5 ; Wang, Zhi-wen 1,2,3; Huang, Yu 1,2,3,4,5; Jian, Ji-chang 1,4; Lu, Yi-shan 1,2,3,4,5
	2020-06-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	101 页码:205-215
通讯作者	Wang, Bei(wong19820204@126.com) ; Lu, Yi-shan(fishdis@163.com)
摘要	The interaction between CD2-associated protein (CD2AP) and CD2 plays a vital role in lymphocyte adhesion and T cells activation in mammals. In this study, a CD2AP gene (GenBank accession number: MK579862; designated as On-CD2AP) was identified from tilapia (Oreochromis niloticus). Sequence analysis showed that On-CD2AP protein shares high similarity with mammals, including three Src homology 3 (SH3) domains, a section of poly proline motif and a coiled coil region. Transcription levels of On-CD2AP were detected in nine tissues of healthy Nile tilapia, and the highest expression levels were detected in the spleen and gill. On-CD2AP were significantly up-regulated in thymus, head kidney and brain after infected by Streptococcus agalactiae, as well as in head kidney leukocytes (HKLs) with LPS and LTA stimulation. Moreover, a section conserved pro-rich motif that are responsible for binding of CD2 to CD2AP were found in the CD2 cytoplasmic sequence of Nile tilapia (On-CD2C). A weak interaction between On-CD2AP and On-CD2C was proved by yeast two-hybrid assay. In addition, the recombinant proteins of CD2AP-His (rOn-CD2AP-His) and GST-CD2C (GST-rOn-CD2C) were obtained through prokaryotic expression system. His pull-down assay showed that rOn-CD2AP-His and GST-rOn-CD2C could bind to each other. These findings indicate that CD2AP is crucial in immune response during S.agalactiae infection, and the mechanism of interaction between CD2AP and CD2 is conservative in Nile tilapia.
关键词	CD2-associated protein Nile tilapia CD2 Immune response T cells activation Streptococcus agalactiae
DOI	10.1016/j.fsi.2020.03.054
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31702386] ; International Cooperation Science & Technology Planning Project of Guangdong Province of China[2017A050501037] ; Shenzhen Science and Technology Project[JCYJ20180306173022502]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000531054100022
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167422
专题	实验海洋生物学重点实验室
通讯作者	Wang, Bei; Lu, Yi-shan
作者单位	1.Guangdong Ocean Univ, Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen Inst, Shenzhen 518120, Guangdong, Peoples R China 2.Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Southern Marine Sci & Engn Guangdong Lab Zhanjian, Fisheries Coll, Zhanjiang, Peoples R China 3.Key Lab Control Dis Aquat Econ Anim Guangdong Hig, Zhanjiang, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Yuan,Wang, Bei,Wang, Zhi-wen,et al. Molecular cloning, characterization and expression profiles of CD2AP in Nile tilapia (Oreochromis niloticus) responding to Streptococcus agalactiae infection and interaction with CD2 cytoplasmic segment[J]. FISH & SHELLFISH IMMUNOLOGY,2020,101:205-215.
APA	Li, Yuan,Wang, Bei,Wang, Zhi-wen,Huang, Yu,Jian, Ji-chang,&Lu, Yi-shan.(2020).Molecular cloning, characterization and expression profiles of CD2AP in Nile tilapia (Oreochromis niloticus) responding to Streptococcus agalactiae infection and interaction with CD2 cytoplasmic segment.FISH & SHELLFISH IMMUNOLOGY,101,205-215.
MLA	Li, Yuan,et al."Molecular cloning, characterization and expression profiles of CD2AP in Nile tilapia (Oreochromis niloticus) responding to Streptococcus agalactiae infection and interaction with CD2 cytoplasmic segment".FISH & SHELLFISH IMMUNOLOGY 101(2020):205-215.

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