Changes in RNA, DNA, protein contents and growth of turbot Scophthalmus maximus larvae and juveniles

doi:10.1111/j.1095-8649.2010.02692.x

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	Changes in RNA, DNA, protein contents and growth of turbot Scophthalmus maximus larvae and juveniles
	Tong, X. H.1,2; Liu, Q. H.1; Xu, S. H.1; Li, J.1 ; Xiao, Z. Z.1; Ma, D. Y.1; Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China
	2010-08-01
发表期刊	JOURNAL OF FISH BIOLOGY
ISSN	0022-1112
卷号	77 期号:3 页码:512-525
文章类型	Article
摘要	The growth potential of turbot Scophthalmus maximus larvae and juveniles was studied using nucleic acid-based indices and protein variables. The experiment was carried out from 4 to 60 days post hatching (dph). A significant increase in instantaneous growth rate during metamorphosis and retarded growth rate during post-metamorphic phase were observed. Ontogenetic patterns of DNA, RNA and protein all showed developmental stage-specific traits. The RNA:DNA ratio decreased up to 12 dph, then increased rapidly till 19 dph and fluctuated until 35 dph followed by a decline to the end. The RNA:DNA ratio was positively correlated with growth rate of juveniles during the post-metamorphic phase, whereas this ratio was not a sensitive indicator of growth during the pre-metamorphic phase and metamorphosis. The protein:DNA ratio showed a similar tendency to the RNA:DNA ratio. Changes of DNA content and protein:DNA ratio revealed that growth of S. maximus performed mainly by hyperplasia from 4 to 12 dph and hypertrophy until 21 dph during the pre-metamorphic larval phase. Growth was dominantly hypertrophical from the early- to mid-metamorphosing phase and hyperplastic thereafter. The results show that the DNA content and protein:DNA ratio can evaluate growth rates of larval and juvenile S. maximus on a cellular level.; The growth potential of turbot Scophthalmus maximus larvae and juveniles was studied using nucleic acid-based indices and protein variables. The experiment was carried out from 4 to 60 days post hatching (dph). A significant increase in instantaneous growth rate during metamorphosis and retarded growth rate during post-metamorphic phase were observed. Ontogenetic patterns of DNA, RNA and protein all showed developmental stage-specific traits. The RNA:DNA ratio decreased up to 12 dph, then increased rapidly till 19 dph and fluctuated until 35 dph followed by a decline to the end. The RNA:DNA ratio was positively correlated with growth rate of juveniles during the post-metamorphic phase, whereas this ratio was not a sensitive indicator of growth during the pre-metamorphic phase and metamorphosis. The protein:DNA ratio showed a similar tendency to the RNA:DNA ratio. Changes of DNA content and protein:DNA ratio revealed that growth of S. maximus performed mainly by hyperplasia from 4 to 12 dph and hypertrophy until 21 dph during the pre-metamorphic larval phase. Growth was dominantly hypertrophical from the early- to mid-metamorphosing phase and hyperplastic thereafter. The results show that the DNA content and protein:DNA ratio can evaluate growth rates of larval and juvenile S. maximus on a cellular level.
关键词	Metamorphosis Rna:Dna Ratio
学科领域	Fisheries ; Marine & Freshwater Biology
DOI	10.1111/j.1095-8649.2010.02692.x
URL	查看原文
收录类别	SCI
语种	英语
WOS记录号	WOS:000280643900005
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/1673
专题	实验海洋生物学重点实验室
通讯作者	Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Tong, X. H.,Liu, Q. H.,Xu, S. H.,et al. Changes in RNA, DNA, protein contents and growth of turbot Scophthalmus maximus larvae and juveniles[J]. JOURNAL OF FISH BIOLOGY,2010,77(3):512-525.
APA	Tong, X. H..,Liu, Q. H..,Xu, S. H..,Li, J..,Xiao, Z. Z..,...&Li, J, Chinese Acad Sci, Ctr Biotechnol R&D, Inst Oceanol, Qingdao 266071, Peoples R China.(2010).Changes in RNA, DNA, protein contents and growth of turbot Scophthalmus maximus larvae and juveniles.JOURNAL OF FISH BIOLOGY,77(3),512-525.
MLA	Tong, X. H.,et al."Changes in RNA, DNA, protein contents and growth of turbot Scophthalmus maximus larvae and juveniles".JOURNAL OF FISH BIOLOGY 77.3(2010):512-525.

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