Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei

doi:10.1016/j.dci.2020.103713

IOCAS-IR > 实验海洋生物学重点实验室

	Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei
	Zhang, Haofang1,2,3 ; Li, Shihao 1,2,4; Wang, Fuxuan 1; Xiang, Jianhai1,2 ; Li, Fuhua 1,2,4,5
	2020-08-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	109 页码:9
摘要	Leucine-rich repeat (LRR) is a vital structure in some pattern recognition receptors such as TLRs, NLRs and newly reported LRR-containing proteins. Apart from some limited reported LRR-containing proteins, most of LRR proteins, especially immune-related proteins, remain uncharacterized functionally. In the present study, a transmembrane protein containing several LRR motifs, designated as LvLRRm, was identified from the shrimp Litopenaeus vannamei. LvLRRm contained a long signal peptide, one LRRNT region, 12 LRR motifs, one LRRCT region and a transmembrane region. The transcripts of LvLRRm were widely distributed in all tested tissues of shrimp and they were responsive to Vibrio parahaemolyticus infection in several immune-related tissues including Oka, intestine, gill and hemocytes. Knockdown of LvLRRm by dsRNA interference led to a decreased survival rate of shrimp infected by Vibrio parahaemolyticus and an increased in vivo Vibrio propagation. Meanwhile, knockdown of LvLRRm also down-regulated the expression levels of genes involved in antibacterial immune signaling pathways, including the transcription factors LvDorsal and LvRelish, and several antimicrobial peptides. These data suggested that LvLRRm played important roles in shrimp against Vibrio infection, which was probably functioning through activation of antibacterial immune signaling pathways. The present study provided new evidence to elucidate the immune function of LRR-containing proteins in invertebrates.
关键词	Leucine-rich repeat RNA interference Vibrio parahaemolyticus Innate immunity Signaling pathway
DOI	10.1016/j.dci.2020.103713
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31772880] ; National Natural Science Foundation of China[31830100] ; National Natural Science Foundation of China[41776158] ; China Agriculture Research system-48[CARS-48]
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000532168100017
出版者	ELSEVIER SCI LTD
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/167374
专题	实验海洋生物学重点实验室
通讯作者	Li, Shihao; Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Wuhan, Hubei, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Zhang, Haofang,Li, Shihao,Wang, Fuxuan,et al. Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,109:9.
APA	Zhang, Haofang,Li, Shihao,Wang, Fuxuan,Xiang, Jianhai,&Li, Fuhua.(2020).Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,109,9.
MLA	Zhang, Haofang,et al."Identification and functional study of an LRR domain containing membrane protein in Litopenaeus vannamei".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 109(2020):9.

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